Purified anti-γ-protocadherin (pan reactive) Antibody (Previously Covance catalog# MMS-5172)

Pricing & Availability
Clone
N159/5 (See other available formats)
Other Names
Protocadherin gamma-A1, PCDH-gamma-A1
Previously
Covance Catalog# MMS-5172
Isotype
Mouse IgG1
Ave. Rating
0 reviews
N159slash5_Purified_g-protocadherin_Antibody_WB_022615
Western blot showing Pan-Gamma-protocadherin-Constant (N159/5) specificity. Lane 1: Molecular Weight Standard; Lane 2: Rat Brain Membrane Lysate; Lane 3: BSA negative control.
  • N159slash5_Purified_g-protocadherin_Antibody_WB_022615
    Western blot showing Pan-Gamma-protocadherin-Constant (N159/5) specificity. Lane 1: Molecular Weight Standard; Lane 2: Rat Brain Membrane Lysate; Lane 3: BSA negative control.
Cat # Size Price Quantity Avail. Save
831701 100 µl $140.00
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Description

Purified anti-γ-protocadherin is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Gamma-protocadherin-A1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:100 - 1:1,000*
 

Application Notes

This antibody is effective in immunoblotting (WB).

*Expected MW: 100 kD

WB Positive Control: Rat Brain Membrane Lysate

The antibody is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). It was subsequently purified using protein G.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen WV, et al. 2012. Neuron. 75:402.
Publication Library
RRID
AB_2564949 (BioLegend Cat. No. 831701)

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Go To Top Version: 2    Revision Date: 06/23/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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