Purified anti-γ-protocadherin-B2 Antibody (Previously Covance catalog# MMS-5140)

Pricing & Availability
Clone
N148/30 (See other available formats)
Other Names
Protocadherin gamma-B2, 3' RACE product, protocadherin gamma B2, PCDH-gamma-B2
Previously
Covance Catalog# MMS-5140
Isotype
Mouse IgG1
Ave. Rating
0 reviews
Product Citations
publications
N148slash30_Purified_Gamma-protocadherin-B2_Antibody_WB_121917
Western blot of anti-γ-protocadherin-B2 antibody (clone N148/30) and mouse IgG1 isotype control. Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 10 µg/mL of clone N148/30 or mouse IgG1 overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat# 405301). Enhanced chemiluminescence was used as the detection system.
  • N148slash30_Purified_Gamma-protocadherin-B2_Antibody_WB_121917
    Western blot of anti-γ-protocadherin-B2 antibody (clone N148/30) and mouse IgG1 isotype control. Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 10 µg/mL of clone N148/30 or mouse IgG1 overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat# 405301). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
832701 100 µl $140.00
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Description

Protocadherins are a group of self adhering molecules belonging to the cadherin superfamily of proteins.  They are predominantly expressed in neurons in widely diverse array. Unlike cadherin proteins, protocadherins do not bind to intracellular catenin molecules.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 719-804 (variable cytoplasmic domain) of mouse gamma-protocadherin-B2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

This antibody is more reactive with human lysates than mouse or rat. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lobas MA, et al. 2011. J. Neurochem. 913-27
Publication Library
RRID
AB_2564959 (BioLegend Cat. No. 832701)

Antigen Details

Structure
Protocadherin gamma-B2 is a 931 amino acid protein with an apparent molecular mass of 100 kD.
Distribution

Cellular distribution: Plasma membrane

Antigen References

1. Prasad T and Weiner JA. 2011. Front Mol. Neurosci. 4:54

Gene ID
93700 View all products for this Gene ID
UniProt
View information about Gamma-protocadherin-B2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/26/2018

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