- Clone
- N148/30 (See other available formats)
- Other Names
- Protocadherin gamma-B2, 3' RACE product, protocadherin gamma B2, PCDH-gamma-B2
- Previously
-
Covance Catalog# MMS-5140
- Isotype
- Mouse IgG1
- Ave. Rating
- 0 reviews
-
Western blot of anti-γ-protocadherin-B2 antibody (clone N148/30) and mouse IgG1 isotype control. Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human brain membrane lysate; Lane 3, 7: 20 µg of mouse brain membrane lysate; Lane 4, 8: 20 µg of rat brain membrane lysate. The blots were incubated with 10 µg/mL of clone N148/30 or mouse IgG1 overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat# 405301). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 832701 | 100 µl | $140.00 | ||||
Protocadherins are a group of self adhering molecules belonging to the cadherin superfamily of proteins. They are predominantly expressed in neurons in widely diverse array. Unlike cadherin proteins, protocadherins do not bind to intracellular catenin molecules.
Product DetailsProduct Details
- Reactivity
- Human, Mouse, Rat
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 719-804 (variable cytoplasmic domain) of mouse gamma-protocadherin-B2.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB).
The antibody cross reacts with a ~45 kD protein in MBM extracts.
WB Positive Control: Human, mouse and rat brain membrane lysate.
This antibody is more reactive with human lysates than mouse or rat. -
Application References
(PubMed link indicates BioLegend citation) -
- Lobas MA, et al. 2011. J. Neurochem. 913-27
- RRID
-
AB_2564959 (BioLegend Cat. No. 832701)
Antigen Details
- Structure
- Protocadherin gamma-B2 is a 931 amino acid protein with an apparent molecular mass of 100 kD.
- Distribution
-
Cellular distribution: Plasma membrane
- Antigen References
-
1. Prasad T and Weiner JA. 2011. Front Mol. Neurosci. 4:54
- Gene ID
- 93700 View all products for this Gene ID
- UniProt
- View information about Gamma-protocadherin-B2 on UniProt.org
Related FAQs
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.