Purified anti-GABRB3 Antibody (Previously Covance catalog# MMS-5134)

Pricing & Availability
Clone
N87/25 (See other available formats)
Other Names
Gamma-aminobutyric acid receptor subunit beta-3, cleft palate 1, GABA(A) receptor subunit beta-3, gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3
Previously
Covance Catalog# MMS-5134
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
N87slash25_PUREAF_GABRB3_Antibody_1_WB_020518
Western blot of purified anti-GABRB3 antibody (clone N87/25). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate; Lane 5: 20ug Jurkat cell lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N87slash25_PUREAF_GABRB3_Antibody_1_WB_020518
    Western blot of purified anti-GABRB3 antibody (clone N87/25). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate; Lane 5: 20ug Jurkat cell lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N87slash25_PUREAF_GABRB3_Antibody_2_IHC-F_020518
    IHC staining of purified anti-GABRB3 antibody (clone N87/25) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N87slash25_PUREAF_GABRB3_Antibody_3_IHC-F_020518
    IHC staining of purified anti-GABRB3 antibody (clone N87/25) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
818501 100 µl $140.00
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Description

Gamma-aminobutyric acid receptor subunit beta-3 (GABRB3) is a component of the hetero-pentameric receptor for gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate brain. The receptor is a ligand-gated multi-subunit chloride channel. The GABRB3 protein is encoded by the GABRB3 gene in humans. Mutations in this gene have been associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, epilepsy and autism.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 370-433 of mouse GABA(A) Receptor, Beta-3 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunohistochemistry, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564821 (BioLegend Cat. No. 818501)

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Go To Top Version: 3    Revision Date: 12/04/2018

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