Purified anti-g protocadherin A (pan reactive) Antibody (Previously Covance catalog# MMS-5171)

Pricing & Availability
Clone
N144/32 (See other available formats)
Other Names
Protocadherin gamma-A3, PCDH-gamma-A3
Previously
Covance Catalog# MMS-5171
Isotype
Mouse IgG2b
Ave. Rating
0 reviews
N144slash32_Purified_g_protocadherin_A_Antibody_1_030118
Western blot of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate; Lane 5: 20 µg of embryonic mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N144slash32_Purified_g_protocadherin_A_Antibody_1_030118
    Western blot of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate; Lane 5: 20 µg of embryonic mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N144slash32_Purified_g_protocadherin_A_Antibody_2_030415
    IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N144slash32_Purified_g_protocadherin_A_Antibody_3_030118
    IHC Image Figure Legend: Human Brain Tissue IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N144slash32_Purified_g_protocadherin_A_Antibody_4_030118
    IHC Image Figure Legend: Rat Brain Tissue IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N144slash32_Purified_g_protocadherin_A_Antibody_5_030118
    IHC Image Figure Legend: Rat Brain Tissue IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
819201 100 µl $140.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Protocadherins are a group of self adhering molecules belonging to the cadherin superfamily of proteins.  They are predominantly expressed in neurons in widely diverse array. Unlike cadherin proteins, protocadherins do not bind to intracellular catenin molecules.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma protocadherin-A3.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg per ml. For immunohistochemistry on paraffin-embedded tissue, a concentration range of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

RRID
AB_2564828 (BioLegend Cat. No. 819201)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03/01/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account