- Clone
- N144/32 (See other available formats)
- Other Names
- Protocadherin gamma-A3, PCDH-gamma-A3
- Previously
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Covance Catalog# MMS-5171
- Isotype
- Mouse IgG2b
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Western blot of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate; Lane 5: 20 µg of embryonic mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-g protocadherin A (pan reactive) antibody (clone N144/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Avail. | Save | ||
---|---|---|---|---|---|---|
819201 | 100 µL | $140 |
Protocadherins are a group of self adhering molecules belonging to the cadherin superfamily of proteins. They are predominantly expressed in neurons in widely diverse array. Unlike cadherin proteins, protocadherins do not bind to intracellular catenin molecules.
Product DetailsProduct Details
- Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma protocadherin-A3.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
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WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 5.0 - 10.0 µg/mL. For immunohistochemistry on paraffin-embedded tissue, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2564828 (BioLegend Cat. No. 819201)
Antigen Details
- Structure
- Expected MW: 100 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Gene ID
- 93711 View all products for this Gene ID
- UniProt
- View information about g protocadherin A on UniProt.org
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