Purified anti-FOXP3 Antibody

Pricing & Availability
Clone
Poly6238 (See other available formats)
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6238_FOXP3_Purified_Antibody_WB_012114.jpg
Western blot analysis of extracts from 293T(lane 1) and 293T transfected with mFOXP3(lane 2) using anti-FOXP3 antibody(poly6238).
  • poly6238_FOXP3_Purified_Antibody_WB_012114.jpg
    Western blot analysis of extracts from 293T(lane 1) and 293T transfected with mFOXP3(lane 2) using anti-FOXP3 antibody(poly6238).
Cat # Size Price Quantity Avail. Save
623801 50 µl (5 Western blots) $135
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623802 200 µl (20 Western blots) $300
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Clones 206D & MF-14 are available as alternatives.
Description

The forkhead/winged helix transcription factor FOXP3 is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. The Poly6238 antibody recognizes both human and mouse FOXP3 and has been shown to be useful for Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted at -20°C.
Application

WB - Quality tested
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. If primary CD4+CD25+ cells are used, it is recommended that 0.5-1 x 106 cell equivalents be loaded per lane. For immunohistochemical staining of paraffin-embedded sections, the suggested use of this reagent is 1:20 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Sato E, et al. 2005. P. Natl. Acad. Sci. USA 102:18538. (IHC) PubMed
  2. Mayack SR and Berg LJ. 2006. J. Immunol. 176:2059. (WB)
  3. Gasper-Smith N, et al. 2006. J. Immunol. 177:4670. (WB)
  4. Tal Guy, et al. 2006. J. Clin. Invest. 7:2022-2032. Pubmed
  5. Thomas AA, et al. 2015. Neuro Oncol. 17:801. PubMed
Product Citations
  1. Sato E, et al. 2005. Proc Natl Acad Sci U S A. 102:18538. PubMed
  2. Zanin-Zhorov A, et al. 2006. J Clin Invest. 116:2022. PubMed
  3. Cohen-Sfady M, et al. 2005. J Immunol. 175:3594. PubMed
  4. Thomas A, et al. 2015. Neuro Oncology. 17:801. PubMed
  5. Qian D, et al. 2017. Clin Cancer Res. 23(2):399-406. PubMed
  6. Lemdani K, et al. 2019. Oncoimmunology. 8:1550342. PubMed
  7. Li F, et al. 2019. Cancer Res. 79(15). PubMed
Publication Library
RRID
AB_315971 (BioLegend Cat. No. 623801)
AB_315972 (BioLegend Cat. No. 623802)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
T cells
Biology Area
Cell Biology, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.

Regulation
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/21/2014

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