Purified anti-FGF-19 Antibody

Pricing & Availability
Clone
13D9C80 (See other available formats)
Other Names
Fibroblast growth factor 19
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
13D9C80_PURE_FGF-19_Antibody_WB_100115
20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 13D9C80_PURE_FGF-19_Antibody_WB_100115
    20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
681102 100 µg $245
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

FGF-19 belongs to a subfamily of the fibroblast growth factors (FGFs) called endocrine FGFs, which consists of FGF-19, FGF-21, and FGF-23. Unlike other subfamily of FGFs, FGF-19 has a low binding affinity to heparin sulfate and functions as a circulating hormone with effects on distant tissues. FGF-19 has reduced binding affinity to the FGF receptors and the interaction requires two necessary cofactors that are α-Klotho and β-Klotho. In the ileum, FGF-19 expression is initiated by bile acids-induced activation of farnesoid X receptor (FXR). In response to bile acid stimulation, FGF-19 is secreted and enters the portal venous circulation, which subsequently down-regulates hepatic bile acid synthesis through repression of the CYP7A1 gene encoding cholesterol 7-alpha-monooxygenase. The impaired regulation of bile acids by FGF-19 leads to bile acid diarrhea also known as idiopathic bile acid malabsorption. FGF-19 has also been demonstrated to regulate carbohydrate and lipid metabolism.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human FGF-19 recombinant protein (25-216 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566443 (BioLegend Cat. No. 681102)

Antigen Details

Structure
Growth factor. 216 amino acids with a predicted molecular weight of 24 kD.
Distribution
Secreted.
Function
FGF-19 functions as a hormone, regulating homeostasis of bile acid, glucose, lipids, phosphate, and vitamin D.
Interaction
Interacts with heparian, α-Klotho, and β-Klotho.
Ligand/Receptor
FGFR1, FGFR2, FGFR3, and FGFR4.
Biology Area
Cell Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Xie MH, et al. 1999. Cytokine 11:729.
2. Harmer NJ, et al. 2004. Biochemistry 43:629.
3. Goetz R, et al. 2007. Mol. Cell Biol. 27:3417.
4. Wu X, et al. 2008. J. Biol. Chem. 283:33304.
5. Song KH, et al. 2009. Hepatology 49:297.
6. Turner N, et al. 2010. Nat. Rev. Cancer 10:116.

Gene ID
9965 View all products for this Gene ID
UniProt
View information about FGF-19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/01/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account