Purified anti-E. coli RNA Sigma 70 Antibody

Pricing & Availability
Clone
2G10 (See other available formats)
Other Names
RNA pol sigma 70, rpoD, sigma D, RNA Polymerase
Previously
NeoClone Catalog# WP004
Isotype
Mouse IgG2b
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Product Citations
publications
2G10_PURE_E.coliRNASigma70_Antibody_WB_092116
Western blot analysis of 50ng of recombinant E. coli RNA Sigma 70 or 15 µg total lysates from E. coli BL21, E. coli DH5α and E. coli Rosetta using anti-E. coli RNA Sigma 70 antibody (clone 2G10) followed by HRP goat anti-mouse IgG antibody .
  • 2G10_PURE_E.coliRNASigma70_Antibody_WB_092116
    Western blot analysis of 50ng of recombinant E. coli RNA Sigma 70 or 15 µg total lysates from E. coli BL21, E. coli DH5α and E. coli Rosetta using anti-E. coli RNA Sigma 70 antibody (clone 2G10) followed by HRP goat anti-mouse IgG antibody .
Cat # Size Price Quantity Avail. Save
663202 100 µg $275
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Description

RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.

Product Details
Technical data sheet

Product Details

Reactivity
E. coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis, Rhodobacter sphaeroides, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Streptococcus faecalis, Arthrobacter globiformis
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store lyophilized antibody at -20°C. After reconstitution, store at 4°C. May be sub-aliquoted and stored at -20°C to avoid multiple freeze thaw cycles.
Application

WB - Quality tested
ELISA - Validated
FA, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# WP004 from Neoclone.

Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Breyer MJ, et al. 1997. J. Bacterial 179:1404.
  2. Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
  3. Strickland MS, et al. 1988. Biochemistry 27:5755.
Product Citations
  1. Schumacher M, et al. 2016. J Biol Chem. 291: 2485 - 2498. PubMed
  2. Saak C, Zepeda-Rivera M, and Gibbs K. 2017. PLoS One. 10.1371/journal.pone.0184797. PubMed
Publication Library
RRID
AB_2564410 (BioLegend Cat. No. 663202)

Antigen Details

Structure
Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947567 View all products for this Gene ID
UniProt
View information about Sigma 70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/21/2016

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