Purified anti-Elongin B Antibody

Pricing & Availability
Clone
10C4 (See other available formats)
Other Names
RNA polymerase II transcription factor SIII p18 subunit, transcription elongation factor B polypeptide 2, TCEB2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10C4_PURE_ElonginB_Antibody_WB_062618
Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1), 1 µg/mL (1:500 dilution, lane 2) and 2 µg/mL (1:250 dilution, lane 3) purified anti-Elongin B antibody, clone 10C4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Elongin B antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 10C4_PURE_ElonginB_Antibody_WB_062618
    Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1), 1 µg/mL (1:500 dilution, lane 2) and 2 µg/mL (1:250 dilution, lane 3) purified anti-Elongin B antibody, clone 10C4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Elongin B antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
629302 100 µg $195
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Description

Elongin B (also known as RNA polymerase II transcription factor SIII p18 subunit and transcription elongation factor B polypeptide 2) is a member of the UbH family containing a ubiquitin-like domain. Elongin functions as a subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. Clone 10C4 recognizes the human elongin B protein and is useful for Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sato Y, et al. 2009. PLoS One. 5:e1000530. PubMed
Publication Library
RRID
AB_2201267 (BioLegend Cat. No. 629302)

Antigen Details

Structure
UbH family, ubiquitin-like domain, heterotrimer; Human Elongin B has two isoforms with predicted MW of 13kD and 18kD
Distribution

Nuclear

Function
Regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites
Interaction
Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Duan D, et al. 1995. Science. 269:1402.
2. Stebbins C, et al. 1999. Science. 284:455.
3. Brower C, et al. 2002. P. Natl. Acad. Sci. USA 99:10353.
4. DeRenzo C, et al. 2003. Nature. 424:685.

Gene ID
6923 View all products for this Gene ID
UniProt
View information about Elongin B on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 06/26/2018

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