Purified anti-Dopamine Receptor D4 Antibody

Pricing & Availability
Clone
2B9/DRD4 (See other available formats)
Other Names
DRD4, Dopamine D4 receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_2B9slashDRd4_PURE_Dopamine_Receptor_Antibody_WB_072617
Western blot of anti-Dopamine Receptor D4 antibody (clone 2B9/DRD4) or mouse IgG1 isotype control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate; Lane 3: 10 ng of recombinant human DRD1 protein. Blots were incubated with 1 µg/ml of primary antibody or 5 µg/ml of mouse IgG1 overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • A_2B9slashDRd4_PURE_Dopamine_Receptor_Antibody_WB_072617
    Western blot of anti-Dopamine Receptor D4 antibody (clone 2B9/DRD4) or mouse IgG1 isotype control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate; Lane 3: 10 ng of recombinant human DRD1 protein. Blots were incubated with 1 µg/ml of primary antibody or 5 µg/ml of mouse IgG1 overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • B_2B9slashDRd4_PURE_Dopamine_Receptor_Antibody_IHC_072617
    IHC staining of anti-Dopamine Receptor D4 antibody (clone 2B9/DRD4) on Substantia Nigra of formalin-fixed paraffin-embedded human Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
Cat # Size Price Quantity Avail. Save
850101 25 µg $115
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850102 100 µg $295
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Description

Dopamine Receptor D4 (DRD4) is a G-protein coupled receptor which inhibits adenyl cyclase activity. It is linked to many neurological and psychiatric conditions including schizophrenia and bipolar disorder, addictive behaviors, Parkinson’s disease, and eating disorders such as anorexia nervosa. DRD4 mutations have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A peptide corresponding to the 3rd extracellular domain of DRD4 (a.a. 176-185).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

IHC result in mouse is much weaker than in human at 5 µg/mL. 

This antibody does not cross react with DRD1 in Western Blot.

Application References

(PubMed link indicates BioLegend citation)
  1. Wolstencroft EC, et al. 2007. J. Mol. Histol. 38(4):333. (WB, IF)
RRID
AB_2715868 (BioLegend Cat. No. 850101)
AB_2715869 (BioLegend Cat. No. 850102)

Antigen Details

Structure
Dopamine Receptor D4 is a 467 amino acids protein with a molecular mass of ~48 kD.
Distribution

Tissue distribution: Mainly in the Central Nervous System. Highly expressed in Retina.
Cellular distribution: Mainly at plasma membrane.

Function
DRD4 is activated by the neurotransmitter dopamine. It is responsible for neuronal signaling in the mesolimbic system. DRD4 inhibits adenylyl cyclase, and has been reported to modulate the circadian rhythm of contrast sensitivity via modulating the rhythmic expression of NPAS2 in the retinal ganglion cells.
Interaction
DRD4 forms homo- and hetero-oligomers with Dopamine Receptor D2, which may modulate agonist-induced downstream signaling. DRD4 may also interact with ADORA2A and KLHL12.
Ligand Receptor
Dopamine.
Cell Type
Dopaminergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Pappa I, et al. 2015. Neurosci. Biobehav. Rev. 57:175.
2. Yildirim BO, Derksen JJ. 2015. Psychiatry Res. 229(3):633-77.

Gene ID
1815 View all products for this Gene ID
UniProt
View information about Dopamine Receptor D4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/28/2017

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