Purified anti-Cytokeratin 8 Antibody

Pricing & Availability
Clone
1E8 (See other available formats)
Other Names
Keratin, type II cytoskeletal 8, CK-8, keratin-8, cytokeratin-8, K8
Isotype
Mouse IgG2a, λ
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Product Citations
publications
1E8_PURE_Cytokeratin8_Antibody_1_IHC_051716
IHC staining of anti-cytokeratin 8 antibody (clone 1E8) on formalin-fixed paraffin-embedded normal human skin. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 1E8_PURE_Cytokeratin8_Antibody_1_IHC_051716
    IHC staining of anti-cytokeratin 8 antibody (clone 1E8) on formalin-fixed paraffin-embedded normal human skin. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 1E8_PURE_Cytokeratin8_Antibody_2_IF_041717
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization, rehydration, antigen retrieval, and blocking. Tissue was stained with purified anti-Cytokeratin 8 antibody (clone 1E8, red). Nuclei were counterstained with DAPI (blue).
  • 1E8_PURE_Cytokeratin8_Antibody_2_WB_051716
    Western blot of anti-cytokeratin 8 antibody (clone 1E8). Lane 1: Molecular Weight Marker; Lane 2: HEPG2 lysate; Lane 3: A431 lysate. The blot was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature, followed by incubation with a fluorescent labeled goat anti-mouse secondary antibody.
  • 1E8_PURE_Cytokeratin8_Antibody_4_WB_011918
    Total lysates (15 µg protein) from HaCaT (Positive control) and THP-1 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti- Cytokeratin 8 antibody, clone 1E8. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # Size Price Quantity Avail. Save
904804 100 µg $215
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Description

Cytokeratin 8 copolymerizes with cytokeratin 18 to form a component of the cytoskeleton in simple epithelia and many epithelial cell derived cancers. It is a member of the Type II keratin family clustered on the long arm of chromosome 12.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Cross Reactivity: Dog, Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against the 12 carboxy-terminal amino acids of HK-8 preceded by a cysteine, CKLVSESSDVLPK.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 5.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy4.

Application References

(PubMed link indicates BioLegend citation)
  1. Memarzadeh S, et al. 2010. Proc. Natl. Acad. Sci. USA 107:17298. PubMed
  2. Hembrough TA. 1996. J. Biol. Chem. 271:25684. PubMed
  3. Hübner A, et al. 2012. Proc. Natl. Acad. Sci. USA 109:12046. PubMed
  4. Easter SL, et al. 2014. PLoS One 9(11):e113247. (IF) PubMed
RRID
AB_2616821 (BioLegend Cat. No. 904804)

Antigen Details

Structure
Cytokeratin 8 is a 483 amino acid protein with a molecular mass of 54 kD.
Distribution

Tissue distribution: colon, small intestine, pancreas, liver, stomach, and salivary glands.
Cellular distribution: extracellular and nucleus.

Function
Cytokeratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Together with KRT19, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3856 View all products for this Gene ID
UniProt
View information about Cytokeratin 8 on UniProt.org

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Go To Top Version: 1    Revision Date: 05/17/2016

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