- 7H8.2C12 (See other available formats)
- Other Names
- Cyt c
- Mouse IgG2a, κ
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- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|612503||50 µl (5 Western blots)||$90|
|612504||200 µl (20 Western blots)||$250|
Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 7H8.2C12 monoclonal antibody recognizes cytochrome-c from most species and has been shown to be useful for Western blotting.Product Details
- Most Species
- Antibody Type
- Host Species
- Horse cyt c-OVA
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 3.0 µg/ml (1:150-1:500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. (WB)
- Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (WB)
- Product Citations
AB_2090157 (BioLegend Cat. No. 612503)
AB_2292697 (BioLegend Cat. No. 612504)
- Heme binding domain; 15 kD
Mitochondrial intermembrane space
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
1. Liu X, et al. 1996. Cell 86:147.
2. Li P, et al. 1997. Cell 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
- Gene ID
- 54205 View all products for this Gene ID
- View information about Cytochrome c on UniProt.org
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