Purified anti-CD243 (ABCB1) Antibody (Previously Covance catalog# SIG-38740)

Pricing & Availability
Clone
4E3.16 (See other available formats)
Other Names
MDR-1, Multidrug resistance protein 1, P-glycoprotein 1, colchicin sensitivity, doxorubicin resistance
Previously
Signet Catalog# 8740-01
Covance Catalog# SIG-38740
Isotype
Mouse IgG2a, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
919401 1 ml $659
 
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Description

CD243 (also known as ABCB1, MDR-1, and P-glycoprotein 1) is an ATP-dependent protein found in the cell membrane, and acts as an efflux pump to transport various substances across the cell membrane and out of cells. CD243 is a member of the MDR/TAP family, whose members are involved in the development of multidrug resistance. CD243 also acts as a transporter in the blood-brain barrier.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a multidrug-resistant human squamous lung cancer cell line SW-1573/500 (Doxorubicin resistant).
Formulation
Phosphate-buffered solution with BSA + 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC
IP - Reported in the literature

Recommended Usage

The optimal working dilution should be determined for each specific assay condition.

Application Notes

This antibody is effective in immunoprecipitation (IP) and immunostaining.

The 4E3.16 monoclonal antibody recognizes a cell surface epitope of the 170 kD multidrug resistance P-glycoprotein. 4E3.16 does not cross-react with the mdr3 gene product. Elevated levels of P-glycoprotein have been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias/lymphomas. It is able to recognize both the glycosylated and non-glycosylated forms of P-glycoprotein.

Application References

(PubMed link indicates BioLegend citation)
  1. Muller MR, et al. 2000. Int. J. Clin. Pharmacol. Ther. 38:180. (FC)
  2. Dhooge C, et al. 1999. Avd. Exp. Med. Biol. 457:11. (ICC)
  3. Huet S, et al. 1998. Cytometry. 34:248. (FC)
  4. Den Boer ML, et al. 1997. Leukemia. 11:1078. (ICC, FC)
  5. Arceci RJ, et al. 1993. Cancer Res. 53:310.
  6. Arceci RJ, et al. 1992. Proc. Am. Assoc. for Cancer Res. 33:471. (IP, IHC)
  7. Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC, ICC, FC) PubMed
Product Citations
  1. Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed
Publication Library
RRID
AB_2565210 (BioLegend Cat. No. 919401)

Antigen Details

Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
CD Molecules
Gene ID
5243 View all products for this Gene ID
UniProt
View information about CD243 (ABCB1) on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 10/19/2017

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