Purified anti-CD18 Antibody (Previously Covance catalog# MRT-5214)

Pricing & Availability
Clone
C71/16 (See other available formats)
Other Names
Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527
Previously
Covance Catalog# MRT-5214
Isotype
Rat Ig2a
Ave. Rating
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Product Citations
publications
C71slash16_Purified_CD18_Antibody_FC_022615
CD18 (C71/16) staining of mouse lymphocytes analyzed by flow cytometry.
  • C71slash16_Purified_CD18_Antibody_FC_022615
    CD18 (C71/16) staining of mouse lymphocytes analyzed by flow cytometry.
Cat # Size Price Quantity Avail. Save
830501 100 µl $234
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Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against cell membrane glycoproteins derived from T-cell lymphoma BW5147 cells.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

FC

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

The optimal working dilution should be determined for each specific assay condition.
FC: 1:1000 - 1:10,000*

Application Notes

This antibody is effective in flow cytometry (FC).

*FC Positive Control: Mouse blood

Rat IgG2a Isotype antibody available: Catalog number: 910601

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. Molecular Complexity of Leukocyte Surface Glycoproteins Related to the Macrophage Differentiation Antigen MAC-1. J. Exp. Med. 154: 1517-1524, November 1981.
RRID
AB_2564939 (BioLegend Cat. No. 830501)

Antigen Details

Biology Area
Cell Biology, Complement, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/01/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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