Purified anti-CD106 (VCAM-1) Antibody

Pricing & Availability
Clone
A16047A (See other available formats)
Other Names
INCAM-110, VCAM1, VCAM 1, Vascular cell adhesion protein 1, V-CAM 1,
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16047A_PURE_CD106_VCAM-1_Antibody_062017
Total cell lysates (15µg protein) from HUVEC and HUVEC treated with recombinant Human TNF-α (10 ng/ml, 16 h) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:25000 (0.02µg/ml), 1:50000 (0.01µg/ml) and 1:250000 (0.002µg/ml) purified anti-CD106 (VCAM-1) antibody (clone A16047A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A16047A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder, M* indicates longer exposure.
  • A16047A_PURE_CD106_VCAM-1_Antibody_062017
    Total cell lysates (15µg protein) from HUVEC and HUVEC treated with recombinant Human TNF-α (10 ng/ml, 16 h) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:25000 (0.02µg/ml), 1:50000 (0.01µg/ml) and 1:250000 (0.002µg/ml) purified anti-CD106 (VCAM-1) antibody (clone A16047A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A16047A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder, M* indicates longer exposure.
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698201 25 µg $85
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698202 100 µg $215
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Description

CD106 is a glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). 

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant VCAM-1/FC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.002 - 0.1 µg/mL (1:5000-1:250000 diluted). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687276 (BioLegend Cat. No. 698201)
AB_2687285 (BioLegend Cat. No. 698202)

Antigen Details

Structure
Ig superfamily, 81 kD. Six N-glycosylation sites, apparent Mw is 90-110kD.
Distribution

Plasma membrane of bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Important in cell-cell recognition. Function in leukocyte-endothelial cell adhesion.
Interaction
Leukocytes
Ligand/Receptor
VLA-4 (α41 integrin) and LPAM-1 (α47 integrin)
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leuko. Biol. 57:168.
3. Bevilacqua MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
7412 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 06/20/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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