Purified anti-Caspase-11 Antibody

Pricing & Availability
Clone
Cas11.17D9 (See other available formats)
Other Names
Casp11
Isotype
Rat IgG2a
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Product Citations
publications
cas11.17D9_caspase-11_Purified_Antibody_WB_012114.jpg
Western blot analysis of Raw264.7 cells starved overnight(lane 1), Raw264.7 cells starved overnight and treated with 1 µg/ml LPS for nine hours (lane 2) and NIH3T3 untreated cells (lane 3) using anti-Caspase11 antibody (clone cas11.17D9).
  • cas11.17D9_caspase-11_Purified_Antibody_WB_012114.jpg
    Western blot analysis of Raw264.7 cells starved overnight(lane 1), Raw264.7 cells starved overnight and treated with 1 µg/ml LPS for nine hours (lane 2) and NIH3T3 untreated cells (lane 3) using anti-Caspase11 antibody (clone cas11.17D9).
Cat # Size Price Quantity Avail. Save
647201 25 µg $100
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647202 100 µg $250
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Description

Murine caspase-11 is an upstream protease of caspase-1 and interacts with the proform of caspase-1 which in turn activates IL-1 beta. Caspase-11 is not constitutively expressed in most cell types. Its expression can be upregulated by LPS, IFN-gamma, and other pro-inflammatory stimuli. NF-κB and STAT1 were reported to be involved in the activation. Caspase-11 knock -out mice were resistant to endotoxin shock and do not produce IL-1 after stimulation with LPS. Their production of IFN-gamma was also deficient.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
His tagged bacterial fusion protein of p 30 of caspase 11
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): 1 to 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schmidt RL, et al. 2012. PLoS One. 7:e45186. PubMed.
Product Citations
  1. Khameneh H, et al. 2011. PLoS One. 6:e23025. PubMed
  2. Min L, et al. 2012. J Immunol. 188:1789. PubMed
  3. Schmidt R, Lenz L 2012. PLoS One. 7:e45186. PubMed
RRID
AB_1937283 (BioLegend Cat. No. 647201)
AB_1937281 (BioLegend Cat. No. 647202)

Antigen Details

Structure
This murine caspase 11 has pro-enzymes a 38 and 43 kD. The activated form consists of p30 and p10.
Distribution
Not constitutively expressed in most cell types, but can be induced by different pro-inflmmatory stimuli including LPS.
Function
Essential for the activation of caspase-1, thus involved in the maturation of IL-1beta and IL-18. Both are important mediators of inflammation.
Interaction
Caspase-1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Lee J et al. 2001. J. Biol. Chem. 276:32956
2. Schauvliege R et al. 2002. J. Biol. Chem. 277:41624
3. Wang S et al. 1998. Cell 92:501

Gene ID
201161 View all products for this Gene ID
UniProt
View information about Caspase-11 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/21/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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