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Purified anti-c-Fos Antibody

Pricing & Availability
Clone
Poly6414 (See other available formats)
Regulatory Status
RUO
Other Names
Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
1_Poly6414_Purified_c-Fos_Antibody_WB_090916
Total cell lysates (15 µg protein) from HT29, HCT116 and Raw264.7 treated with PMA 100ng/mL for one hour or without treatment were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-c-Fos (clone poly6414). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH Antibody was used as a loading control.
  • 1_Poly6414_Purified_c-Fos_Antibody_WB_090916
    Total cell lysates (15 µg protein) from HT29, HCT116 and Raw264.7 treated with PMA 100ng/mL for one hour or without treatment were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-c-Fos (clone poly6414). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH Antibody was used as a loading control.
  • 2_Poly6414_Purified_c-Fos_Antibody_WB_032119
    Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with a 1:1000 dilution of Purified anti-c-Fos Antibody, clone Poly6414, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP anti-rabbit IgG Antibody (Cat. No. 410406) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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641401 25 µL $112
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641402 100 µL $265
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Description

The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 - 1:4000 antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Fan W, et al. 2009. Brain Res Red. 59:324. PubMed
  2. Sugimoto K, et al. 2014. Neurosci Res. PubMed
Product Citations
  1. Chung H, et al. 2021. Nat Methods. 18:1204. PubMed
  2. Sugimoto K, et al. 2015. Neurosci Res. 96: 20-29. PubMed
  3. Fan W, et al. 2009. Brain Res Rev. 59:324. PubMed
RRID
AB_2106632 (BioLegend Cat. No. 641401)
AB_2106629 (BioLegend Cat. No. 641402)

Antigen Details

Structure
c-fos heterodimerizes with c-jun to form the transcription factor AP-1; contains a bZIP region with a DNA-binding domain and a leucine zipper domain; approximately 62 kD.
Distribution

Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes.

Function
FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal form
Interaction
Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Modification
Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Dony C, et al. 1987. Nature 328:711.
2. Grigoriadis AE, et al. 1994. Science 266:443.
3. Glover JNM, et al. 1995. Nature 373:257.

Regulation
c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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