Purified anti-C/EBP β (3 isoforms C/EBP β, LAP, LIP) Antibody

Pricing & Availability
Clone
1H7 (See other available formats)
Other Names
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1H7_Purified_CEBP-B_Antibody_1_032019
Total cell lysates (15 µg total protein) from THP-1 (negative control), Raw264.7, P388D1 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-cEBP β Antibody, clone 1H7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1H7_Purified_CEBP-B_Antibody_1_032019
    Total cell lysates (15 µg total protein) from THP-1 (negative control), Raw264.7, P388D1 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of Purified anti-cEBP β Antibody, clone 1H7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
606202 100 µg $185
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Description

C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination. The 1H7 monoclonal antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence of mouse and rat C/EBP β, LIP, and LAP isoforms. The 1H7 antibody does not recognize human C/EBP β, LIP or LAP.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant LIP
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP, IP, IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Recognizes three mouse isoforms: C/EBP β, LAP, LIP. Does not cross-react with human.

Application References

(PubMed link indicates BioLegend citation)
  1. Su, et al. 2003. J. Biol. Chem. 278:51150. (ChIP)
  2. Blidner AG, et al. 2015. J Immunol. 194:3452. PubMed
Product Citations
  1. Blidner A, et al. 2015. J Immunol. 194:3452. PubMed
Publication Library
RRID
AB_315675 (BioLegend Cat. No. 606202)

Antigen Details

Structure
C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively
Distribution

Nuclear

Function
Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
Interaction
CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
Modification
Phosphorylation, Ubiquitination
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642.
2. Eaton E, et al. 2001. J. Cell Physiol. 189:91.
3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557.
4. Hattori T, et al. 2003. Oncogene. 22:1273.

Regulation
Growth hormone
Gene ID
12608 View all products for this Gene ID 25301 View all products for this Gene ID
UniProt
View information about C/EBP beta on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 03/20/2019

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