Purified anti-Brevican Antibody (Previously Covance catalog# MMS-5127)

Pricing & Availability
Clone
N294A/6 (See other available formats)
Other Names
Brevican core protein, BEHAB, brain-enriched hyaluronan-binding protein, brevican (brain specific proteoglycan in the aggrecan family)
Previously
Covance Catalog# MMS-5127
Isotype
Mouse IgG2b
Ave. Rating
0 reviews
N294Aslash6_Purified_Brevican_Antibody_1_WB_030415
Western blot of anti-Brevican antibody (clone N294A/6). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N294Aslash6_Purified_Brevican_Antibody_1_WB_030415
    Western blot of anti-Brevican antibody (clone N294A/6). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N294Aslash6_Purified_Brevican_Antibody_2_IHC_030415
    IHC staining of anti-Brevican antibody (clone N294A/6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N294Aslash6_Purified_Brevican_Antibody_3_IHC_030415
    IHC staining of anti-Brevican antibody (clone N294A/6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
820101 100 µl $140.00
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Description

Brain-specific proteoglycan in the aggrecan family (brevican), also known as brain-enriched hyaluronan-binding protein (BEHAB), is a brain chondroitin sulfate proteoglycan specifically expressed in the central nervous system. It plays a role in development and formation of the brain extracellular matrix and may be involved in malignancy of brain tumor cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 219-655 of rat Brevican.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 5-10 μg/ml
IHC: 1-10 μg/ml

Tissue: Formalin-fixed Paraffin-embedded
Pre-treatment: Citrate
Primary incubation: 24 hours at 4°C

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Expected MW: 140 kD (proteolyticaly cleaved fragment of Brevican may be observed at ~50 kD in some tissue lysates)

WB Positive Control: Rat Brain Membrane Lysate

IHC Positive Control: Rat and mouse brain. This antibody does not cross-react with human brevican protein.

RRID
AB_2564837 (BioLegend Cat. No. 820101)

Related FAQs

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Go To Top Version: 3    Revision Date: 10/12/2017

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