Purified anti-Brevican Antibody (Previously Covance catalog# MMS-5127)

Pricing & Availability
Clone
N294A/6 (See other available formats)
Other Names
Brevican core protein, BEHAB, brain-enriched hyaluronan-binding protein, brevican (brain specific proteoglycan in the aggrecan family)
Previously
Covance Catalog# MMS-5127
Isotype
Mouse IgG2b
Ave. Rating
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Product Citations
publications
N294Aslash6_Purified_Brevican_Antibody_1_WB_030415
Western blot of anti-Brevican antibody (clone N294A/6). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N294Aslash6_Purified_Brevican_Antibody_1_WB_030415
    Western blot of anti-Brevican antibody (clone N294A/6). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N294Aslash6_Purified_Brevican_Antibody_2_IHC_030415
    IHC staining of anti-Brevican antibody (clone N294A/6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N294Aslash6_Purified_Brevican_Antibody_3_IHC_030415
    IHC staining of anti-Brevican antibody (clone N294A/6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • N294Aslash6_Purified_Brevican_Antibody_4_IHC_062118
    IHC staining of purified anti-Brevican antibody (clone N294A/6) on frozen rat brain tissue. The tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 conjugated Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
820101 100 µl $140.00
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Description

Brain-specific proteoglycan in the aggrecan family (brevican), also known as brain-enriched hyaluronan-binding protein (BEHAB), is a brain chondroitin sulfate proteoglycan specifically expressed in the central nervous system. It plays a role in development and formation of the brain extracellular matrix and may be involved in malignancy of brain tumor cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 219-655 of rat Brevican.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P, IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded, a concentration range of 1.0 - 10 µg/ml is suggested. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P) and immunohistochemical staining on frozen tissue sections (IHC-F).

Expected MW: 140 kD (proteolyticaly cleaved fragment of Brevican may be observed at ~50 kD in some tissue lysates)

RRID
AB_2564837 (BioLegend Cat. No. 820101)

Antigen Details

Structure
Brevican is a 911 amino acid protein with a molecular mass of ~ 99 kD.
Gene ID
25393 View all products for this Gene ID
UniProt
View information about Brevican on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 06/25/2018

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