Purified anti-β-Dystroglycan Antibody

Pricing & Availability
Clone
7D11 (See other available formats)
Other Names
Beta-dystroglycan, Beta-DG
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_7D11_PURE_betaDystro_Antibody_5_121918.png
Western blot of purified anti-β-Dystroglycan antibody (clone 7D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_7D11_PURE_betaDystro_Antibody_5_121918.png
    Western blot of purified anti-β-Dystroglycan antibody (clone 7D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 7D11_PURE_betaDystro_Antibody_1_082417
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 7D11_PURE_betaDystro_Antibody_2_121918_updated.png
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 7D11_PURE_betaDystro_Antibody_3_121918.png
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 7D11_PURE_betaDystro_Antibody_4_121918.png
    IHC staining of purified anti-β-Dystroglycan antibody (clone 7D11) on formalin-fixed paraffin-embedded mouse heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
850201 25 µg $80
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850202 100 µg $205
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Description

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and Utrophin scaffolds axin to the cytoskeleton. β-dystroglycan also has been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated
IHC-F, ICC - Reported in Literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Praissman JL, et al. 2016. Elife. E14473 (ICC)
  2. Vasquez-Limeta A, et al. 2014. PLoS One. 9(3):e90629 (WB)
  3. Martinez-Vieyra IA, et al. 2013. biochim Biophys Acta. 1833(3):698 (ICC, WB)
  4. Beedle AM, et al. 2012. J Clin Invest. 122(9):3330 (IHC-F, WB)
  5. Hu H, et al. 2011. Neurosci Lett. 489(1): 10 (WB)
RRID
AB_2715870 (BioLegend Cat. No. 850201)
AB_2715871 (BioLegend Cat. No. 850202)

Antigen Details

Structure
Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
Distribution

Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS.
Cellular distribution: nucleus, plasma membrane, and cytoskeleton.

Function
β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Interaction
Interacts with Dystrophin, Utrophin and other cytoskeletal components such as axin.
Ligand Receptor
Receptor for Dystrophin and Utrophin
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Murphy S, et al. 2015. Comput Struct Biotechnol J. 14:20-7
2. Moore CJ, et al. 2012. Neuromuscul Disord. 22(11): 959

Gene ID
1605 View all products for this Gene ID
UniProt
View information about beta-Dystroglycan on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 12/19/2018

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