Purified anti-β-Dystroglycan Antibody

Pricing & Availability
Clone
4F7 (See other available formats)
Other Names
Beta-dystroglycan, Beta-DG, DAG1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
4F7_PURE_beta-Dystroglycan_Antibody_1_WB_053017
Western blot of purified anti-β-Dystroglycan antibody (clone 4F7), and isotype-matched mouse IgG2b control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate. The blots were incubated with 5 µg/ml of clone 4F7 or mouse IgG overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The antibody also detects a smaller band, slightly higher than the 25 kD molecular marker, which corresponds to a cleavage product of β-dystroglycan as previously reported.
  • 4F7_PURE_beta-Dystroglycan_Antibody_1_WB_053017
    Western blot of purified anti-β-Dystroglycan antibody (clone 4F7), and isotype-matched mouse IgG2b control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate. The blots were incubated with 5 µg/ml of clone 4F7 or mouse IgG overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The antibody also detects a smaller band, slightly higher than the 25 kD molecular marker, which corresponds to a cleavage product of β-dystroglycan as previously reported.
  • 4F7_PURE_beta-Dystroglycan_Antibody_2_IHP_053017
    IHC staining of purified anti-β-Dystroglycan antibody (clone 4F7) on formalin-fixed, paraffin-embedded normal human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 4F7_PURE_beta-Dystroglycan_Antibody_3_IHP_053017
    IHC staining of purified anti-β-Dystroglycan antibody (clone 4F7) on formalin-fixed, paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
849401 25 µg $80
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849402 100 µg $205
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Description

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and utrophin scaffolds axin to the cytoskeleton. β-dystroglycan has also been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sumita K, et al. 2007. J. Neurochem. 100(1):154. (IP, WB)
RRID
AB_2687292 (BioLegend Cat. No. 849401)
AB_2687293 (BioLegend Cat. No. 849402)

Antigen Details

Structure
Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
Distribution

Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS.

Cellular distribution: nucleus, plasma membrane, and cytoskeleton.

Function
β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Interaction
Interacts with dystrophin, utrophin and other cytoskeletal components such as axin.
Ligand Receptor
Receptor for dystrophin and utrophin.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules
Antigen References

1. Murphy S and Ohlendieck K. 2015. Comput. Struct. Biotechnol. J. Nov 26;14:20-7.
2. Moore CJ and Winder SJ. 2012. Neuromuscul. Disord. Nov: 22(11): 959.

Gene ID
1605 View all products for this Gene ID
UniProt
View information about beta-Dystroglycan on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 05/30/2017

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