Purified anti-β-Amyloid, 17-24 Antibody (Previously Covance catalog# SIG-39220)

Pricing & Availability
Clone
4G8 (See other available formats)
Workshop
HCDM listed
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9220-02
Signet Catalog# 9220-05
Signet Catalog# 9220-10
Covance Catalog# SIG-39220
Isotype
Mouse IgG2b, κ
Ave. Rating
4 reviews
Product Citations
publications
4G8_Pure_Beta-Amyloid_Ascites_Antibody_1_070218
IHC staining of purified anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G8_Pure_Beta-Amyloid_Ascites_Antibody_1_070218
    IHC staining of purified anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G8_Pure_Beta-Amyloid_Ascites_Antibody_2_070218
    IHC staining of purified anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4G8_Pure_Beta-Amyloid_Ascites_Antibody_3_070218
    Direct ELISA of purified anti-β-Amyloid, 17-24 (clone 4G8) antibody binding to the plate-immobilized human Aβ1-40, human Aβ1-42, rodent Aβ1-42 peptides, and recombinant human APP751 protein. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # Size Price Quantity Avail. Save
800712 25 µl $95
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800701 200 µl $472
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800702 500 µl $923
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800703 1 ml $1,519
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Description

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
Direct ELISA - Validated
IHC-F, ICC, IP - Reported in Literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.0 µg/ml is suggested. For Direct ELISA applications, a concentration range of 0.15 - 0.5 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residues 17-24 of β amyloid. The epitope lies within amino acids 18-22 of β amyloid (VFFAE). 4G8 β-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms.

This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections (IHC-F) and immunocytochemistry (ICC)

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed
  2. Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed
  3. Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed
  4. Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed
  5. Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed
  6. Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP)
  7. Fonte V, et al. 2002. PNAS. 110:4853 (IP)
Product Citations
  1. Lutzenberger M, et al. 2015. PLoS One. 10: 0134228. PubMed
  2. Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed
  3. Lian H, et al. 2016. J Neurosci. 36: 577 - 589. PubMed
  4. Kretner B, et al. 2016. EMBO Mol Med. 10.15252/emmm.201505952. PubMed
  5. Keable A, et al. 2016. Biochim Biophys Acta. 1862:1037-1046. PubMed
  6. Sherman M, et al. 2016. J Neurosci. 36: 9647 - 9658. PubMed
  7. A Kimura, S Hata, T Suzuki 2016. J Biol Chem. 291: 24041 - 24053. PubMed
  8. Liu L, et al. 2017. Sci Rep. 7:40467. PubMed
  9. Cacciottolo M, et al. 2017. Transl Psychiatry. 7:e1022. PubMed
  10. Sau-Yeen Loke, Peter Tsun-Hon Wong, Wei-Yi Ong 2017. Neurochem Int. 102:33-56. PubMed
  11. Eimer WA 2018. Neuron. 99:56. PubMed
  12. Wu Y, et al. 2019. Nat Commun. 10:58. PubMed
  13. Chiang ACA, et al. 2018. Am J Pathol. 188:739. PubMed
  14. Osborne C, et al. 2018. J Cell Sci. 131:. PubMed
  15. Garcia–Esparcia P, et al. 2017. Front Neurol. 8:89. PubMed
  16. Finke JM, et al. 2017. Biochim Biophys Acta. 1861:2228. PubMed
  17. González C, et al. 2018. Mol Psychiatry. 23:2363. PubMed
  18. Zhang J, et al. 2019. Aging Cell. 18:e12978. PubMed
  19. Zhao P, et al. 2019. Front Behav Neurosci. 13:138. PubMed
  20. Prpar Mihevc S, et al. 2019. Front Neurosci. 13:604. PubMed
  21. Kim S, et al. 2019. Int J Mol Sci. 20. PubMed
  22. Harper MM, et al. 2019. Invest Ophthalmol Vis Sci. 60:2716. PubMed
  23. Ezpeleta J, et al. 2019. Nat Commun. 10:3442. PubMed
  24. Wang B, et al. 2017. Mol Psychiatry. 22:990. PubMed
  25. Jonson M, et al. 2018. Cell Chem Biol. 25:595. PubMed
Publication Library
RRID
AB_2734548 (BioLegend Cat. No. 800712)
AB_2564633 (BioLegend Cat. No. 800701)
AB_2564634 (BioLegend Cat. No. 800702)
AB_662812 (BioLegend Cat. No. 800703)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83.
  4. Puig KL, et al. 2012. Exp Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595.
  6. Walsh DM, et al.  2007. J Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 17-24 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07/02/2018

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