- Poly6221 (See other available formats)
- Other Names
- Actin, cytoplasmic 1
- Rabbit Polyclonal IgG
- Ave. Rating
- 2 reviews
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|622101||50 µl (5 Western blots)||$100|
|622102||200 µl (20 Western blots)||$250|
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.Product Details
- Mouse, Rat, Human
- Antibody Type
- Host Species
- Peptide-KLH, NH2 terminus
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- The antibody was purified by affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
WB - Quality tested
IF, IHC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
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- Toltlt LJ,et al. 2008.J. Immunol. 181:2165. PubMed
- Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. PubMed
- Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
- Rahman MK, et al. 2010. J. Immunol. 184:7247. PubMed
- Shikama Y, et al. 2011. Innate Immun. 17:3. PubMed
- Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:70. PubMed
- Piao X, et al. 2012. Sci Signal. 5:93. PubMed
- Ng CS, et al. 2013. J. Virol. 87:9511. PubMed
- Harvey BS, et al. 2014. Cytokine. 65:236. PubMed
- Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
- Hua X, et al. 2015. PloS One. 10:128039. PubMed
- Product Citations
AB_315945 (BioLegend Cat. No. 622101)
AB_315946 (BioLegend Cat. No. 622102)
- Member of the actin family; 42 KD
Ubiquitously expressed in the cytoplasm of all eukaryotic cells
- Actins are highly conserved proteins that are involved in cell motility
- Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers, Western Blot Controls
- Molecular Family
- Antigen References
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
- Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
- Gene ID
- 11461 View all products for this Gene ID
- View information about beta-actin on UniProt.org
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