Purified anti-β-actin Antibody

Pricing & Availability
Clone
Poly6221 (See other available formats)
Other Names
Actin, cytoplasmic 1
Isotype
Rabbit Polyclonal IgG
Ave. Rating
2 reviews
Product Citations
publications
poly6221_PURE_betaactin_Antibody_1_WB_090616
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_1_WB_090616
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG cells (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β-actin antibody (Poly6221). Proteins were visualized using an HRP Donkey anti-rabbit IgG Antibody and chemiluminescence detection.
  • poly6221_PURE_betaactin_Antibody_2_IF_062016
    Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
See supplemental IHC data...
Cat # Size Price Quantity Avail. Save
622101 50 µl (5 Western blots) $100
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622102 200 µl (20 Western blots) $250
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH, NH2 terminus
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IF, IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.PubMed
  4. KishidaT,et al.2007.J. Immunol. 179:8554.PubMed
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. PubMed
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. PubMed
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. PubMed
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  9. Rahman MK, et al. 2010. J. Immunol. 184:7247. PubMed
  10. Shikama Y, et al. 2011. Innate Immun. 17:3. PubMed
  11. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:70. PubMed
  12. Piao X, et al. 2012. Sci Signal. 5:93. PubMed
  13. Ng CS, et al. 2013. J. Virol. 87:9511. PubMed
  14. Harvey BS, et al. 2014. Cytokine. 65:236. PubMed
  15. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  16. Hua X, et al. 2015. PloS One. 10:128039. PubMed
Product Citations
  1. Joyce C, et al. 2006. J Biol Chem. 281:33053. PubMed
  2. Kishida T, et al. 2007. J Immunol. 179:8554. PubMed
  3. Yanagiya T, et al. 2007. Obesity . 1.022222222. PubMed
  4. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed
  5. Sawada T, et al. 2008. J Biol Chem. 283:26820. PubMed
  6. Toltl L, et al. 2008. J Immunol. 181:2165. PubMed
  7. Ouimet M, et al. 2008. Arterioscler Thromb Vasc Biol. 28:1144. PubMed
  8. Rahman M, et al. 2010. J Immunol. 184:7247. PubMed
  9. Shikama Y, et al. 2011. Innate Immunity. 17:03. PubMed
  10. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:F70. PubMed
  11. Piao X, et al. 2012. Sci Signal. 5:93. PubMed
  12. Ng C, et al. 2013. J Virol. 87:9511. PubMed
  13. Harvey B, et al. 2014. Cytokine. 65:236. PubMed
  14. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  15. Hua X, et al. 2015. PLoS One. 10:128039. PubMed
  16. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  17. Wakatsuki S, et al. 2015. J Cell Biol. 211: 881 - 896. PubMed
  18. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  19. Checa M, et al. 2016. PLoS One. 11: 0150383. PubMed
  20. Takeda K, et al. 2017. Nat Commun. 8:14607. PubMed
  21. Agabiti S, Li J, Wiemer A 2017. Cell Death Dis. 10.1038/cddis.2017.101. PubMed
  22. Grundmann M, et al. 2018. Nat Commun. 9:341. PubMed
  23. Zheng X, et al. 2018. Int J Mol Med. 128:1300. PubMed
  24. Wang D 2018. Immunity. 48:659. PubMed
  25. Merten N 2018. Cell chemical biology. 25:775. PubMed
  26. Panwar P, et al. 2018. Br J Pharmacol. 175:902. PubMed
  27. Kumarasinghe N, et al. 2019. BMC Res Notes. 12:33. PubMed
  28. Tsurusaki S, et al. 2019. Cell Death Dis. 10:449. PubMed
  29. Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed
Publication Library
RRID
AB_315945 (BioLegend Cat. No. 622101)
AB_315946 (BioLegend Cat. No. 622102)

Antigen Details

Structure
Member of the actin family; 42 KD
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Interaction
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers, Western Blot Controls
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Regulation
Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 09/08/2016

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