Purified anti-Bcl11b Antibody

Pricing & Availability
Clone
25B6 (See other available formats)
Other Names
B-cell CLL/lymphoma 11B (zinc finger protein), CTIP2, B-cell lymphoma/leukemia 11B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
25B6_PURE_Bcl11b_Antibody_1_WB_110317
Total lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2, negative control), and EL4 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-Bcl11b Antibody, clone 25B6 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-Bcl11b Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 25B6_PURE_Bcl11b_Antibody_1_WB_110317
    Total lysates (15 µg protein) from Jurkat (lane 1), HeLa (lane 2, negative control), and EL4 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-Bcl11b Antibody, clone 25B6 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rat-IgG secondary antibody conjugated to HRP for the anti-Bcl11b Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 25B6_PURE_Bcl11b_Antibody_2_IF_083016
    Jurkat cells were stained with anti-bcl11b, clone 25B6 (1:200 dilution), and tubulin-α, clone TU-01, followed by DyLight™ 549 conjugated anti-rat IgG and DyLight™ 488 conjugated anti-mouse IgG. Nuclei are stained with DAPI.
Cat # Size Price Quantity Avail. Save
650601 25 µg $150
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650602 100 µg $350
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Description

The transcription factor Bcl11b is expressed in thymus, mainly T cells. It is indispensable for T lineage development. When Bcl11b was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro, and effectively prevented tumor metastasis in vivo. Therefore, ITNKs may represent a new cell source for cell-based therapies.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Mouse, Zebrafish
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Bcl11b
Formulation
This antibody is provided in Hepes-buffered solution, pH 7.5, 0.02% NaN3.
Preparation
Protein G affinity purified.
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB, ICC - Quality tested
CHIP, IHC-P - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and immunofluorescence microscopy. Western blotting, suggested working dilution(s): Use 0.5 µg antibody per ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution of 1:200. The optimal concentration should be determined by titration for each individual assay of interest.

Application References

(PubMed link indicates BioLegend citation)
  1. Tomassy GS, et al. 2010. P. Natl. Acad. Sci. USA 107:3576.
  2. Cherrier T, et al. 2009. Oncogene 28:3380.
  3. Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed.
Product Citations
  1. Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed
Publication Library
RRID
AB_10896795 (BioLegend Cat. No. 650601)
AB_10915967 (BioLegend Cat. No. 650602)

Antigen Details

Structure
894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution

Nucleus

Function
Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction
Interacts with TFCOUP1, SIRT1, ARP1, and EAR2
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Liu P, et al. 2011. Immunol. Rev. 238:138.
2. Ikawa T, et al. 2010. Science 329:93.
3. Li L, et al. 2010. Science 329:89.
4. Li P, et al. 2010. Science 329:85.
5. Tydell CC, et al. 2007. J. Immunol. 179:421.
6. Avram D, et al. 2002. Biochem. J. 368:555.

Gene ID
64919 View all products for this Gene ID
UniProt
View information about Bcl11b on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08/30/2016

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