Purified anti-BACH2 Antibody

Pricing & Availability
Clone
16B10B53 (See other available formats)
Other Names
BTB and CNC homolog 2, Transcription regulator protein BACH2,
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16B10B53_PURE_BACH2_Antibody_WB_031317
Total cell lysates (15µg protein) from THP1 and Raji (negative controls) and Ramos were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-BACH2 antibody (clone 16B10B53) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M*: MW ladder with longer exposure time.
  • 16B10B53_PURE_BACH2_Antibody_WB_031317
    Total cell lysates (15µg protein) from THP1 and Raji (negative controls) and Ramos were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-BACH2 antibody (clone 16B10B53) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M*: MW ladder with longer exposure time.
Cat # Size Price Quantity Avail. Save
695602 100 µg $235
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Description

BTB and CNC Homolog 2 (BACH2) is a transcription factor of the basic leucine zipper family. BACH2 participates in the regulation of both innate and adaptive immune responses. In B cells, BACH2 plays critical roles in somatic hypermutation, class-switch recombination, memory B cell formation, and repression of myeloid genes. In T cells, BACH2 promotes the development of Foxp3+ regulatory T cells and memory T cells. Bach2 is required for the maintenance of naive CD4 T cells by suppressing effector T cell differentiation. BACH2 is also required for the maturation and function of alveolar macrophages. Defects in BACH2 gene are associated with autoimmune and allergic diseases, such as asthma, type 1 diabetes, celiac disease, rheumatoid arthritis, and generalized vitiligo.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BACH2 recombinant protein (513-841 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650879 (BioLegend Cat. No. 695602)

Antigen Details

Structure
841 amino acids with a predicted molecular weight of 92.5 kD. Contains a N-terminal BTB/POZ domain for protein-protein interaction and a C-terminal bZIP domain for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
BACH2 is a transcription factor involved in the development and differentiation of both B and T cells.
Interaction
Forms homodimers or heterodimers with Maf-related transcription factors.
Cell Type
B cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kuwahara M, et al. 2016. Nat. Commun. 7:12596.
2. Sidwell T, et al. 2016. Nat. Immunol. 17:744.
3. Roychoudhuri R, et al. 2016. Nat. Immunol. 17:851.
4. Itoh-Nakadai A, et al. 2014. Nat. Immunol. 15:1171.
5. Kuwahara M, et al. 2014. Nat. Commun. 5:3555.
6. Kim EH, et al. 2014. J. Immunol. 192:985.

Gene ID
60468 View all products for this Gene ID
UniProt
View information about BACH2 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 03/15/2017

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