Purified anti-AU1 Epitope Tag Antibody (Previously Covance catalog# MMS-130P)

Pricing & Availability
Clone
AU1 (See other available formats)
Other Names
AU1 epitope tag, DTYRYI epitope tag, DTYRYI tag
Previously
Covance Catalog# MMS-130P
Isotype
Mouse IgG2a
Ave. Rating
1 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
901905 25 µl $75
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901901 200 µl $303
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901902 500 µl $474
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Description

The AU1 tag, which consists of the peptide sequence DTYRYI, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical data sheet

Product Details

Reactivity
DTYRYI Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody AU1 was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope DTYRYI.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IF, IP, Purification

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000
IF: 1:1,000
IP: 1:150

Application Notes

This antibody is effective in immunofluorescence (IF), immunoblotting (WB), and immunoprecipitation (IP) of tagged proteins.

The clone was specifically designed for use in epitope tagging. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.

Application References

(PubMed link indicates BioLegend citation)
  1. Mothe-Satney I, et al. 2000. J Biol Chem. 275:33836.
  2. Goldstein DJ, et al. 1992. EMBO J. 11:4851.
  3. Lim PS, et al. 1990. J Infect Dis. 162:1263.
  4. Sauter D, et al. 2011. PLoS Pathog. 8:e1003093. (WB, IF)
  5. Kluge SF, et al. 2013. Retrovirology 10:32. (WB, IF) PubMed
  6. Heigele A, et al. 2012. J. Virol. 86:36. WB) PubMed
Product Citations
  1. Heigele A, et al. 2012. J Virol. 86:36-48. PubMed
  2. Kluge S, et al. 2013. Retrovirology. 10:32. PubMed
  3. Waise TMZ, et al. 2019. Nat Commun. 10:714. PubMed
Publication Library
RRID
AB_2734667 (BioLegend Cat. No. 901905)
AB_2565013 (BioLegend Cat. No. 901901)
AB_2565014 (BioLegend Cat. No. 901902)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about AU1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 01/25/2019

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