Purified anti-ATM Phospho (Ser1981) Antibody

Pricing & Availability
Clone
10H11.E12 (See other available formats)
Other Names
Ataxia telangiectasia mutated (ATM) kinase, serine-protein kinase, AT mutated
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10H11.E12_ATM-pS1981_090711
Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-ATM pS1981 (clone 10H11.E12) antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1 - lysates from untreated Hela cells; Lane 2 - lysates from Hela cells treated with UV.
  • 10H11.E12_ATM-pS1981_090711
    Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-ATM pS1981 (clone 10H11.E12) antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1 - lysates from untreated Hela cells; Lane 2 - lysates from Hela cells treated with UV.
  • 10H11.E12_Purified_ATM_Antibody_IF_090214
    HeLa cells were stained with purified anti-ATM Phospho (Ser 1981) (clone 10H11.E12) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled with green. Nuclei were stained with DAPI (blue).
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Cat # Size Price Quantity Avail. Save
651201 25 µg $125
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651202 100 µg $275
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Description

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. ATM is held inactive in unirradiated cells as a dimer or higher-order multimer. Cellular irradiation induces rapid intermolecular autophosphorylation of serine 1981 that causes dimer dissociation and initiates cellular ATM kinase activity. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated synthetic peptide SLAFEEGSpQSTTISS
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IF - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


Patent No. USA: 7,108,992

Product Citations
  1. Bhandaru M, et al. 2015. PLoS One. 10: 0134678. PubMed
  2. Luo Q, et al. 2018. Cell Physiol Biochem. 51:2421. PubMed
Publication Library
RRID
AB_10900815 (BioLegend Cat. No. 651201)
AB_10900251 (BioLegend Cat. No. 651202)

Antigen Details

Structure
3056 amino acids, 350 kD.
Distribution
Primarily nuclear, also found in endocytic vesicles.
Function
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Phospho-Proteins
Antigen References

1. Lee JH and Paull TT 2007. Oncogene 26:7741.
2. Bakkenist CJ and Kastan MB 2003. Nature 421:499.
3. McConville CM, et al. 1996. Am. J. Hum. Genet. 59:320.
4. Tang X, et al. 2008. Mol. Cell Biol. 28:2559.

Gene ID
472 View all products for this Gene ID
UniProt
View information about ATM on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09/02/2014

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