Purified anti-ASIC1 Antibody (Previously Covance catalog# MMS-5261)

Pricing & Availability
Clone
N271/44 (See other available formats)
Other Names
ACCN2, ASIC, BNaC2, Acid-sensing ion channel 1, degenerin 2, brain sodium channel 2, acid sensing ion channel, amiloride-sensitive cation channel 2, neuronal
Previously
Covance Catalog# MMS-5261
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
N271slash44_Purified_ASIC1_Antibody_101917
Western blot of anti-ASIC1 antibody (clone N271/44). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat# 405301). Enhanced chemiluminescence was used as the detection system.
  • N271slash44_Purified_ASIC1_Antibody_101917
    Western blot of anti-ASIC1 antibody (clone N271/44). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat# 405301). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
833501 100 µl $140.00
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Description

Acid-sensing ion channel (ASIC) 1, also known as brain sodium channel (BNaC2), degenerin 2 and amiloride-sensitive cation channel 2 (ACCN2), is a sodium channel activated by extracellular protons that is expressed in nearly all neurons. ASIC1 may play a role in learning, pain transduction, memory development, and fear conditioning.

Product Details
Technical data sheet

Product Details

Reactivity
Human (by Alignment), Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 4-10 µg/ml

Application Notes

This antibody is effective in immunoblotting (WB).

*Expected MW: 60 kD

WB Positive Control: Human, mouse and rat brain membrane lysates. This clone cross-reacts with human ASIC1, however detection level is weaker than that observed with mouse or rat AISC1.

RRID
AB_2564967 (BioLegend Cat. No. 833501)

Related FAQs

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Go To Top Version: 3    Revision Date: 10/19/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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