Purified anti-Ankyrin-B Antibody (Previously Covance catalog# MMS-5247)

Pricing & Availability
Clone
N105/17 (See other available formats)
Other Names
Ankyrin-2, ankyrin B, ankyrin, brain, non-erythroid ankyrin, ankyrin-2 nonerythrocytic
Previously
Covance Catalog# MMS-5247
Isotype
Mouse IgG2a
Ave. Rating
0 reviews
Product Citations
publications
1_N105slash17_PURE_Ankyrin-B_Antibody_WB_121317
Western blot of anti-Ankyrin-B antibody (clone N105/17). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 1_N105slash17_PURE_Ankyrin-B_Antibody_WB_121317
    Western blot of anti-Ankyrin-B antibody (clone N105/17). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 2_N105slash17_PURE_Ankyrin_Antibody_Mouse_121317
    IHC staining of anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • 3_N105slash17_PURE_Ankyrin_Antibody_Human_121317
    IHC staining of anti-Ankyrin-B antibody (clone N105/17) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
821501 100 µl $140.00
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Description

Ankyrins are adaptor proteins that mediate the attachment of membrane proteins to the cytoskeleton. Ankyrin-B (Ankyrin-2) is highly expressed in cardiac tissue where it has an essential role in stabilizing ion transporters and channels in cardiomyocytes in the membrane. Mutations in ankyrin-B are associated with cardiac arrhythmia and ankyrin-B may be involved in human heart failure.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 203-496 (ankyrin repeats 6 to 14) of human Ankyrin-B.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5-10 µg per ml. For immunohistochemistry, a concentration range of 5-10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

WB Positive Control: Human, mouse and rat brain membrane lysates.

IHC Positive Control: Human, mouse and rat brain tissue.

RRID
AB_2564851 (BioLegend Cat. No. 821501)

Related FAQs

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Go To Top Version: 3    Revision Date: 12/13/2017

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