Purified anti-AMIGO-1 Antibody (Previously Covance catalog# MMS-5268)

Pricing & Availability
Clone
L86A/37 (See other available formats)
Other Names
Amphoterin-induced protein 1, alivin-2, amphoterin-induced gene and ORF
Previously
Covance Catalog# MMS-5268
Isotype
Mouse IgG2b
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Product Citations
publications
L86A37_Pure_AMIGO1_Antibody_1_011018
IHC staining of anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • L86A37_Pure_AMIGO1_Antibody_1_011018
    IHC staining of anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • L86A37_Pure_AMIGO1_Antibody_2_011018
    IHC staining of anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4*deg;C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • L86A37_Pure_AMIGO1_Antibody_3_011018
    IHC staining of anti-AMIGO-1 antibody (clone L86A/37) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • L86A37_Pure_AMIGO1_Antibody_4_011018
    Western blot of anti-AMIGO-1 antibody (clone L86A/37). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
822101 100 µl $140.00
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Description

Amphoterin-induced protein 1 (AMIGO-1) is a transmembrane protein containing six LRR domains and one Ig domain and is highly expressed in the mammalian nervous system. It promotes growth and fasciculation of neurites from cultured hippocampal neurons and may be involved in fasciculation as well as myelination of developing neural axons. AMIGO-1 may have a role in regeneration as well as neural plasticity in the adult nervous system.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Mandikian D, et al. 2014. Comp Neurol. 522(15): 3555. (IHC-P)
RRID
AB_2564856 (BioLegend Cat. No. 822101)

Antigen Details

Structure
Amigo-1 has an apparent molecular weight ranging from 60-80 kD.
Distribution

Tissue distribution: Nervous system
Cellular distribution: Plasma membrane

Antigen References

1. Chen Y, et al. 2006. Brain Res Rev. 51(2):265.

Gene ID
57463 View all products for this Gene ID
UniProt
View information about AMIGO-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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