Purified anti-AMIGO-1 Antibody (Previously Covance catalog# MMS-5271)

Pricing & Availability
Clone
L86/36 (See other available formats)
Other Names
Amphoterin-induced protein 1, alivin-2, amphoterin-induced gene and ORF, Ali2, AMIGO and KIAA1163
Previously
Covance Catalog# MMS-5271
Isotype
Mouse IgG1
Ave. Rating
0 reviews
Product Citations
publications
L86Aslash37_Purified_AMIGO1_Antibody_1_101917
IHC staining of anti-AMIGO-1 antibody (clone L86/36) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm
  • L86Aslash37_Purified_AMIGO1_Antibody_1_101917
    IHC staining of anti-AMIGO-1 antibody (clone L86/36) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm
  • L86Aslash37_Purified_AMIGO1_Antibody_2_101917
    IHC staining of anti-AMIGO-1 antibody (clone L86/36) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm
  • L86Aslash37_Purified_AMIGO1_Antibody_3_101917
    IHC staining of anti-AMIGO-1 antibody (clone L86/36) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat# 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat# 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 20 µm
  • L86Aslash37_Purified_AMIGO1_Antibody_4_101917
    Western blot of anti-AMIGO-1 antibody (clone L86/36). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
804801 100 µl $140.00
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Description

Amphoterin-induced protein 1 (AMIGO-1) is a transmembrane protein containing six LRR domains and one Ig domain and is highly expressed in the mammalian nervous system. It promotes growth and fasciculation of neurites from cultured hippocampal neurons and may be involved in fasciculation as well as myelination of developing neural axons. AMIGO-1 may have a role in regeneration as well as neural plasticity in the adult nervous system.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 395-493 (cytoplasmic C-terminus) of human AMIGO-1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

This antibody does not cross-react with Amigo-2 or Amigo-3.

RRID
AB_2564672 (BioLegend Cat. No. 804801)

Antigen Details

Structure
Expected MW: 60 - 80 kD (depending on maturity & glycosylation)
Gene ID
57463 View all products for this Gene ID
UniProt
View information about AMIGO-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/02/2018

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