Purified anti-α-Synuclein Phospho (Tyr39) Antibody

Pricing & Availability
Clone
A15119B (See other available formats)
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Mouse IgG1, κ
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Product Citations
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A15119B_PURE_alpha-Synuclein_phospho_Tyr39_Antibody_1_IHC_011017
IHC staining of α-synuclein deposits with purified anti-α-Synuclein Phospho (Tyr39) antibody (clone A15119B) on formalin-fixed, paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • A15119B_PURE_alpha-Synuclein_phospho_Tyr39_Antibody_1_IHC_011017
    IHC staining of α-synuclein deposits with purified anti-α-Synuclein Phospho (Tyr39) antibody (clone A15119B) on formalin-fixed, paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • A15119B_PURE_alpha-Synuclein_phospho_Tyr39_Antibody_2_ELISA_011017
    Direct ELISA of purified anti-α-Synuclein Phospho (Tyr39) antibody (clone A15119B) binding to plate-immobilized recombinant human unmodified and pY39 and pY125 α-synuclein proteins. ELISA was performed by coating wells with 150 ng of each recombinant α-synuclein protein. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
See high resolution IHC-P/ELISA data...
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849201 25 µg $115
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849202 100 µg $285
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Description

Alpha-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy. Phosphorylation of α-synuclein at Tyr39 may modulate clearance of the protein and contribute to Parkinson’s disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated α-synuclein synthetic peptide containing phospho tyrosine 39.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Direct ELISA - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Direct ELISA, a concentration range of 0.02 - 3.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650704 (BioLegend Cat. No. 849201)
AB_2650702 (BioLegend Cat. No. 849202)

Antigen Details

Structure
α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue distribution: primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein, Phospho-Proteins
Antigen References

1. Mor DE, et al. 2016. Neurobiol Dis. 88:66. PubMed
2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed
3. Bartels T, et al. 2011. Nature. 477(7362): 107. PubMed
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. PubMed
5. Mahul-Mellier AL, et al. 2014. Hum Mol Genet. 23(11):2858. PubMed

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein Phospho (Tyr39) on UniProt.org

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Go To Top Version: 1    Revision Date: 01/23/2017

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