- D8B7 (See other available formats)
- Other Names
- Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1
- Mouse IgG2b, κ
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- Product Citations
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The spectrins are a family of widely distributed cytoskeletal proteins which are involved in actin crosslinking. Spectrin α-chain heterodimerizes with tissue-specific β-chains. It is specifically expressed in nonerythrocytic cells. The protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the gene are the cause of early infantile epileptic encephalopathy. Fodrin, a spectrin that seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.Product Details
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
IHC-P - Quality tested
ICC, WB - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. For Western blotting, the suggested use of this reagent is 0.05 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.
This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.
Reactivity to Drosophila was only validated with the purified format.
- Additional Product Notes
For Western blotting, the suggested use of this reagent is 0.05 - 1 µg/ml in human, mouse, and rat and 0.1 - 2 µg/ml in Drosophila.
(PubMed link indicates BioLegend citation)
- Konieczny P, et al. 2008. J Cell Biol. 181:667-81
- Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
- Xu J, et al. 2000. J Cell Science. 113:3805-14
- De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
- Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
- Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
- Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
- Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
- Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed
AB_2749952 (BioLegend Cat. No. 803205)
AB_2749953 (BioLegend Cat. No. 803206)
- Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Presynaptic proteins, Scaffold Proteins
- Antigen References
- Pike BR, et al. 2001. J Neurochem. 78(6):1297-306.
- Weiss ES, et al. 2009. Ann Thorac Surg. 88(2):543
- Gene ID
- 6709 View all products for this Gene ID
- View information about Alpha-II Spectrin on UniProt.org
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