Purified anti-α-E-Catenin Antibody

Pricing & Availability
Clone
1G5 (See other available formats)
Other Names
Catenin (Cadherin-Associated Protein), Alpha 1, 102kD, Alpha-1-Catenin, Alpha E-Catenin , Cadherin-Associated Protein,102 kD, CAP102, CTNNA1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1G5_PURE_alpha-E-Catenin_Antibody_1_ICC_042319
MeOH fixed HeLa cells were stained with α-E-Catenin (clone 1G5) at 1 µg/ml. Goat-anti mouse Alexa Fluor® 594 was used as a secondary antibody and the cells were counterstained using DAPI.
  • 1G5_PURE_alpha-E-Catenin_Antibody_1_ICC_042319
    MeOH fixed HeLa cells were stained with α-E-Catenin (clone 1G5) at 1 µg/ml. Goat-anti mouse Alexa Fluor® 594 was used as a secondary antibody and the cells were counterstained using DAPI.
  • 1G5_PURE_alpha-E-Catenin_Antibody_2_WB_090915
    30 µg of A431 cell lysate was loaded and subjected to electrophoresis. Membrane was blotted using α-E-Catenin (clone 1G5) at 10 µg/ml followed by a fluorescent labeled goat anti-mouse IgG secondary.
Cat # Size Price Quantity Avail. Save
844801 25 µg $95
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844802 100 µg $235
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Description

α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties. Catenin can associate with both E- and N-cadherins. It was originally thought to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and α-catenin was shown not to bind to F-actin when assembled in the complex suggesting a more complex, dynamic linkage between actin and these adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MBP-alpha catenin fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
WB - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sacco PA, et al. 1995. J. Biol. Chem. 270:20201. (WB, IP, IF)
  2. Johnson KR, et al. 1993. Exp. Cell Res. 2:252.
RRID
AB_2565821 (BioLegend Cat. No. 844801)
AB_2565808 (BioLegend Cat. No. 844802)

Antigen Details

Structure
A 102 kD member of the α-Catenin family composed primarily of alpha helical bundles. It contains three vinculin homology domains, a vinculin binding region, an actin binding domain, and a dimerization domain.
Distribution

Cytoplasm, cytoskeleton, cell junctions, adherens junctions, cell membrane, peripheral membraine protein, and the cytoplasmic side.

Function
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, hemophilic adhesion, signal transduction, Wnt signaling, and cell junction organization.
Interaction
α-catenin binds to β-catenin. Cadherin binds to the p120-catenin and β-catenin. This cadherin-catenin complex interacts with the actin cytoskeleton. α-catenin can also bind vinculin, then associate with actin nucleating proteins.
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Hirano S, et al. 2012. Physiol. Rev. 92:597.

Gene ID
1495 View all products for this Gene ID
UniProt
View information about alpha-E-Catenin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 04/23/2019

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