Purified anti-Akt Phospho (Ser473) Antibody

Pricing & Availability
Clone
Poly6490 (See other available formats)
Other Names
Protein kinase B alpha (PRK-BA, PKB), Serine/Threonine specific kinase RAC alpha, Protein kinase Akt
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6490_PURE_AKTphospho(ser473)_Antibody_1_WB_062116
Western blot analysis of NIH3T3 starved overnight (lane 1) cell, NIH3T3 starved overnight and treated with PDGF 100ng/ml for 15 minutes (lane 2) and Jurkat (lane 3) cells using anti-AKT Phospho (Ser473) antibody (Poly6490). Purified anti-β-actin Antibody (Poly6221) was used as loading control.
  • poly6490_PURE_AKTphospho(ser473)_Antibody_1_WB_062116
    Western blot analysis of NIH3T3 starved overnight (lane 1) cell, NIH3T3 starved overnight and treated with PDGF 100ng/ml for 15 minutes (lane 2) and Jurkat (lane 3) cells using anti-AKT Phospho (Ser473) antibody (Poly6490). Purified anti-β-actin Antibody (Poly6221) was used as loading control.
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Cat # Size Price Quantity Avail. Save
649001 50 µl (5 Western blots) $125
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649002 200 µl (20 Western blots) $350
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Description

Akt (also known as protein kinase B alpha) is a 60 kD serine/threonine specific kinase containing a pleckstrin domain. Akt plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon activation. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase.  Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of Akt at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Activated Akt can then phosphorylate a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3,Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2).  Akt promotes cell survival by inhibiting apoptosis through phosphorylation and by inactivation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex. The phosphor-Akt (S473) antibody specifically detects phosphorlated Akt at the Ser473 site.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified Synthetic peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
This antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IF - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Miyamoto S, et al. 2012. Cancer Res. 72:2609. PubMed
  2. Yin S, et al. 2013. J Biol Chem. 288:26448. PubMed
  3. Duan L, et al. 2014. FEBS Lett. 588:1644. PubMed
Product Citations
  1. Miyamoto S, et al. 2012. Cancer Res. 72:2609. PubMed
  2. Yin S, et al. 2013. J Biol Chem. 288:26448. PubMed
  3. Duan L, et al. 2014. FEBS Lett. 588:1644. PubMed
  4. Saito Y, et al. 2017. Sci Rep. . 10.1038/s41598‐017‐16443‐w. PubMed
Publication Library
RRID
AB_10612760 (BioLegend Cat. No. 649001)
AB_10613474 (BioLegend Cat. No. 649002)

Antigen Details

Structure
Serine/Threonine family of kinases, pleckstrin domain; 60 kD
Distribution
Ubiquitously expressed, translocates to the membrane upon activation.
Function
Catalytically inactive multimeric complex. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
Interaction
Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
Cell Sources
Phosphorylates substrates in cytoplasm, nucleus.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Jacinto E, et al. 2006. Cell 127:125.
2. Inoki K, et al. 2002. Nat. Cell. Biol. 4:648.
3. Franke TF, et al. 1995. Cell 81:727.
4. Brunet A, et al. 1999. Cell 96:857.
5. Yang WL, et al. 2010 Cell Cycle 9:487.

Gene ID
207 View all products for this Gene ID
UniProt
View information about phosphorylated Akt on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/21/2016

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