- Poly6490 (See other available formats)
- Other Names
- Protein kinase B alpha (PRK-BA, PKB), Serine/Threonine specific kinase RAC alpha, Protein kinase Akt
- Rabbit Polyclonal IgG
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- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|649001||50 µl (5 Western blots)||$125|
|649002||200 µl (20 Western blots)||$350|
Akt (also known as protein kinase B alpha) is a 60 kD serine/threonine specific kinase containing a pleckstrin domain. Akt plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon activation. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of Akt at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Activated Akt can then phosphorylate a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3,Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and by inactivation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex. The phosphor-Akt (S473) antibody specifically detects phosphorlated Akt at the Ser473 site.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Modified Synthetic peptide
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- This antibody was purified by affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
WB - Quality tested
IF - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The optimal concentration should be determined by titration for each individual assay of interest.
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
- Product Citations
AB_10612760 (BioLegend Cat. No. 649001)
AB_10613474 (BioLegend Cat. No. 649002)
- Serine/Threonine family of kinases, pleckstrin domain; 60 kD
- Ubiquitously expressed, translocates to the membrane upon activation.
- Catalytically inactive multimeric complex. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
- Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
- Cell Sources
- Phosphorylates substrates in cytoplasm, nucleus.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Signal Transduction
- Molecular Family
- Antigen References
1. Jacinto E, et al. 2006. Cell 127:125.
2. Inoki K, et al. 2002. Nat. Cell. Biol. 4:648.
3. Franke TF, et al. 1995. Cell 81:727.
4. Brunet A, et al. 1999. Cell 96:857.
5. Yang WL, et al. 2010 Cell Cycle 9:487.
- Gene ID
- 207 View all products for this Gene ID
- View information about phosphorylated Akt on UniProt.org
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