Purified anti-AICD Antibody (Previously Covance catalog# SIG-39148)

Pricing & Availability
Clone
Poly8119 (See other available formats)
Other Names
Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9148-005
Covance Catalog# SIG-39148
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly8119_Purified_AICD_Antibody_IF_030315
Immunofluorescence of nuclei in tissue of transgenic mice expressing human APP-PS1delta9 (~9 months) using Clone Poly8119 (red). Other staining in blue is by anti-amyloid beta. Sections were pretreated for antigen retrieval.
  • Poly8119_Purified_AICD_Antibody_IF_030315
    Immunofluorescence of nuclei in tissue of transgenic mice expressing human APP-PS1delta9 (~9 months) using Clone Poly8119 (red). Other staining in blue is by anti-amyloid beta. Sections were pretreated for antigen retrieval.
Cat # Size Price Quantity Avail. Save
811901 50 µg $450
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Description

APP intracellular domain (AICD) is a peptide fragment that results from intramembraneous proteolysis of the amyloid precursor protein (APP). APP is a single-pass transmembrane protein that is involved in the pathophysiology of Alzheimer's disease (AD). APP is cleaved by gamma-secretase that releases the AICD into the cytoplasm. Soon after its release from the membrane, AICD is rapidly degraded in the cytoplasm partly by insulin degrading enzymes.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Do not store antibody diluted below 50 µg/mL in the absence of protein (i.e. add 2% bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ELISA, IHC, WB
IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.

The optimal working dilution should be determined for each specific assay condition.

Application Notes

This antibody is effective in immunohistochemistry (IHC), immunoblotting (WB), and ELISA.

This antibody is specific for AICD (APP intracellular domain), which is a peptide fragment that results from the cleavage of C83 or C99 at the epsilon site. It does not react with the C57 or C59 γ-secretase cleavage products of APP.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Kaori K, Yasushi K, Yasunari M, and Satoki U. Axonal Protection via Modulation of the Amyloidogenic Pathway in Tumor Necrosis Factor-Induced Optic Neuropathy. Invest. Ophthalmol. Vis. Sci.: 53, 7675 -7683, Nov 2012. (WB)
  2. Stieren ES, El Ayadi A, Xiao Y, Siller E, Landsverk ML, Oberhauser AF, Barral JM, and Boehning D. Ubiquilin-1 Is a Molecular Chaperone for the Amyloid Precursor Protein. J. Biol. Chem.: 256, 35689 -35698, Oct 2011. (WB)
  3. Tampellini D, Rahman N, Lin MT, Capetillo-Zarate E, and Gouras GK. Impaired ?-Amyloid Secretion in Alzheimer's Disease Pathogenesis. J. Neurosci.: 31, 15384 - 15390, Oct 2011. (IF) PubMed
Product Citations
  1. Tampellini D, et al. 2011. J Neurosci. 31:15384-15390. PubMed
Publication Library
RRID
AB_2564761 (BioLegend Cat. No. 811901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about AICD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 02/09/2017

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