Purified anti-14-3-3 ε Antibody

Pricing & Availability
Clone
Poly6379 (See other available formats)
Other Names
14-3-3E, YWHAE
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
a-Poly6379_Purified_14-3-3-e_Antibody_1_032119
Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-14-3-3ε Antibody, clone Poly6379, at 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • a-Poly6379_Purified_14-3-3-e_Antibody_1_032119
    Total cell lysates (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-14-3-3ε Antibody, clone Poly6379, at 1:500 dilution overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b-Poly6379_012111
    Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-14-3-3 ε antibody (clone Poly6379). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
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637901 25 µl $95
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Description

14-3-3ε is a 29 kD protein that belongs to the family of 14-3-3 proteins. This family of proteins mediates signal transduction and cell cycle progression by binding to phosphoserine and phosphothreonine motifs on a large number of target molecules. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases as well as protein kinase C. There are eight known isoforms of 14-3-3: alpha, beta, gamma, delta, epsilon, zeta, eta, and theta.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Mouse (weak)
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Full length human 14-3-3 epsilon recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Poly6379 antibody has been shown to be useful for western blotting of human 14-3-3 epsilon protein.

This product may contain other non-IgG subtypes.

This clone may also recognize other 14-3-3 family members.

Product Citations
  1. Cloutier A, et al. 2018. Sci Rep. 8:2206. PubMed
Publication Library
RRID
AB_2217770 (BioLegend Cat. No. 637901)

Antigen Details

Structure
14-3-3 protein superfamily, homodimer, 29 kD
Distribution

Cytoplasm

Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif.
Interaction
BCL2, Calmodulin 1, CDC25A/B, CDK11, IGFIR, IRS1, IRS2, MEKK3, Raf-1, c-Src,
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Jin DY, et al. 1996. Nature 382:308.
2. Conklin DS, et al. 1995. Proc. Natl. Acad. Sci. USA 92:7892.
3. Zannis-Hadjopoulos M, et al. 2008. Trends Biochem. Sci. 33:44.

Regulation
Phosphorylation disrupts dimerization
Gene ID
36059 View all products for this Gene ID
UniProt
View information about 14-3-3e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/21/2019

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