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Precision Count Beads™ can be used to obtain absolute counts of cells or any other samples acquired on a flow cytometer. Precision Count Beads™ are fluorescent particles about 10µm that can be detected across a broad range of wavelengths (405-635nm excitation and 400-800nm emission). Absolute numbers of cells can be determined by comparing the bead count and cell count. Precision Count Beads™ can work with all kinds of assays including a lyse, no-wash whole blood assay.Product Details
- Precision Count Beads™ are supplied in a solution containing 0.02% sodium azide and 0.01% NP40.
- Storage & Handling
- Store between 2°C and 8°C.
FC - Quality tested
- Recommended Usage
Vigorously vortex the Precision Count Beads™ bottle for 30-40 seconds to ensure complete mixing and break up of aggregates that may occur during storage. For flow cytometric analysis, the suggested use of this reagent is 100 µl per test. Please read the Precision Count Beads™ protocol and applications for further instructions.
- Application Notes
Precision Count Beads™ are designed for counting the absolute number of cells in a complex population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm).
- Gene ID