PerCP/Cyanine5.5 anti-mouse IL-22 Antibody

Pricing & Availability
Clone
Poly5164 (See other available formats)
Other Names
Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF
Isotype
Goat Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
Poly5164_PerCpCy5.5_IL-22_Antibody_FC_041714.jpg
PMA/ionomycin-stimulated (6 hours) Th17 polarized BALB/c mouse CD4+ T cells (in the presence of Brefeldin A) were stained with CD4 FITC, then intracellularly stained with IL-22 (clone Poly5164) PerCP/Cyanine5.5.
  • Poly5164_PerCpCy5.5_IL-22_Antibody_FC_041714.jpg
    PMA/ionomycin-stimulated (6 hours) Th17 polarized BALB/c mouse CD4+ T cells (in the presence of Brefeldin A) were stained with CD4 FITC, then intracellularly stained with IL-22 (clone Poly5164) PerCP/Cyanine5.5.
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
516411 100 tests $180
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-22 is a cytokine structurally related to IL-10.  Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD.  It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.  It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells.  AhR activation increases Th17 polarization and induces IL-22 production.  IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ.  IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin.  IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease.  Moreover, it plays a critical role in mucosal immunity and the wound healing process.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard.

In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively.

Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations.

Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended.

Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization.1

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Veldhoen M, et al. 2009. J. Exp. Med. 206:43.
Product Citations
  1. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
Publication Library
RRID
AB_2563373 (BioLegend Cat. No. 516411)

Antigen Details

Structure
A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD.
Distribution

Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.

Function
Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing.
Cell Type
NK cells, Th1, Th17, Th2
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Martin B, et al. 2009. Immunity 31:321.
2. Liang SC, et al. 2006. J. Exp. Med. 203:2271.
3. Veldhoen M, et al. 2008. J. Exp. Med. 206:43.
4. Zheng Y, et al. 2007. Nature 445:648.
5. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
6. Wolk K, et al. 2007. J. Immunol. 178:5973.

Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 09/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account