- Poly5164 (See other available formats)
- Other Names
- Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
IL-22 is a cytokine structurally related to IL-10. Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD. It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells. It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells. AhR activation increases Th17 polarization and induces IL-22 production. IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ. IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin. IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease. Moreover, it plays a critical role in mucosal immunity and the wound healing process.Product Details
- Antibody Type
- Host Species
- Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard.
In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively.
Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations.
Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended.
Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization.1
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Veldhoen M, et al. 2009. J. Exp. Med. 206:43.
- Product Citations
AB_2563373 (BioLegend Cat. No. 516411)
- A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD.
Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.
- Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing.
- Cell Type
- NK cells, Th1, Th17, Th2
- Biology Area
- Molecular Family
- Antigen References
1. Martin B, et al. 2009. Immunity 31:321.
2. Liang SC, et al. 2006. J. Exp. Med. 203:2271.
3. Veldhoen M, et al. 2008. J. Exp. Med. 206:43.
4. Zheng Y, et al. 2007. Nature 445:648.
5. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
6. Wolk K, et al. 2007. J. Immunol. 178:5973.
- Gene ID
- 50929 View all products for this Gene ID
- View information about IL-22 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.