- P1E6-C5 (See other available formats)
- Other Names
- α2 integrin, VLA-2 α chain, Integrin α2 chain
- Mouse IgG1, κ
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- Product Citations
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CD49b is a 170 kD transmembrane protein, also known as α2 integrin, VLA-2 α chain, Integrin α2 and GPIa. It associates with CD29 (β1 integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.Product Details
- Antibody Type
- Host Species
- HT1080 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: in vitro blocking activity1,2, immunoprecipitation3, and ELISA4.
(PubMed link indicates BioLegend citation)
- Hirsch MS, et al. 1997. Dev. Dyn. 210:249. (Block)
- Sawhney RS, et al. 2006. J. Biol. Chem. 281:8497. (Block)
- Lee SA, et al. 2009. Carcinogensis. 30:1872. (IP)
- Zßrate S, et al. 2004. J. Virol. 78:10839. (ELISA)
AB_2820014 (BioLegend Cat. No. 359315)
AB_2820015 (BioLegend Cat. No. 359316)
- Integrin alpha chain family, type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD; associates with CD29 to form collagen, laminin receptor
Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts
- With CD29; binds collagen and laminin
- Forms heterodimer with integrin β1 (CD29) which is a receptor for laminin and collagen
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Kaplan C, et al. 1991. Br. J. Haematol. 78:425.
2. Kiefel V, et al. 1991. Vox Sang. 60:244.
3. Nieuwenhuis HK, et al. 1985. Nature 318:470.
4. Takada Y and Helmer ME. 1989. J. Cell Biol. 109:397.
- Gene ID
- 3673 View all products for this Gene ID
- View information about CD49b on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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