- OX-33 (See other available formats)
- Regulatory Status
- Other Names
- Leukocyte common antigen, high molecular weight form
- Mouse IgG1, κ
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- Product Citations
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CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Leukocyte common antigen purified from rat splenocytes.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Smith T, et al. 2000. Nature Med. 6:62. (IHC)
AB_2565944 (BioLegend Cat. No. 202317)
AB_2565945 (BioLegend Cat. No. 202318)
- Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Primarily on B lymphocytes
- B cell receptor signal transduction
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.
- Gene ID
- 24699 View all products for this Gene ID
- View information about CD45RA on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-rat CD45RA||OX-33||FC, IHC-F|
|Biotin anti-rat CD45RA||OX-33||FC|
|FITC anti-rat CD45RA||OX-33||FC|
|PE anti-rat CD45RA||OX-33||FC|
|Alexa Fluor® 488 anti-rat CD45RA||OX-33||FC|
|APC anti-rat CD45RA||OX-33||FC|
|PerCP/Cyanine5.5 anti-rat CD45RA||OX-33||FC|
|PE/Cyanine7 anti-rat CD45RA||OX-33||FC|
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