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PerCP/Cyanine5.5 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
PK136_PerCPCyanine55_NK11_Antibody_101818
C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) APC and NK1.1 (clone PK136) PerCP/Cyanine5.5 (left), or mouse IgG2a, ? PerCP/Cyanine5.5 isotype control (right).
  • PK136_PerCPCyanine55_NK11_Antibody_101818
    C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) APC and NK1.1 (clone PK136) PerCP/Cyanine5.5 (left), or mouse IgG2a, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
108727 25 µg $93
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108728 100 µg $319
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Minute L, et al. 2020. J Immunother Cancer. 8:. PubMed
  2. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  3. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  4. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  5. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  6. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  7. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  8. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  9. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  10. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  11. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  12. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  13. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  14. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  15. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  16. Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed
  17. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  18. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  19. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  20. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  21. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  22. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  23. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  24. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  25. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  26. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  27. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  28. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  29. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  30. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  31. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  32. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  33. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  34. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  35. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  36. Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed
  37. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  38. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  39. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  40. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  41. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  42. Gawish R, et al. 2022. Elife. 11:. PubMed
  43. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  44. Tian L, et al. 2012. J Autoimmun. 38:39. PubMed
  45. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  46. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID
AB_2132706 (BioLegend Cat. No. 108727)
AB_2132705 (BioLegend Cat. No. 108728)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 3    Revision Date: 10/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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