PerCP/Cyanine5.5 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5-114-15-2_PerCPCyanine55_I-A-I-E_Antibody_101618
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and I-A/I-E (clone M5/114.15.2) PerCP/Cyanine5.5 (left), or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
  • M5-114-15-2_PerCPCyanine55_I-A-I-E_Antibody_101618
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and I-A/I-E (clone M5/114.15.2) PerCP/Cyanine5.5 (left), or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
107625 25 µg $100
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107626 100 µg $275
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Gonçalves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  2. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  3. Gounder AP, et al. 2018. PLoS Pathog. 14:e1007001. PubMed
  4. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  5. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  6. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  7. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  8. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  9. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  10. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  11. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  12. McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed
  13. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  14. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  15. Ji DX, et al. 2019. Nat Microbiol. 1.644444444. PubMed
  16. Movahedi K, et al. 2012. Cancer Res. 74:4165. PubMed
  17. Pello O, et al. 2012. PLoS One. 7:e45399. PubMed
  18. Volkov A, et al. 2013. PLoS One. 8:60565. PubMed
  19. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  20. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  21. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  22. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  23. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  24. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  25. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  26. Bartmann J, et al. 2016. Int Immunol. 28: 473 - 487. PubMed
  27. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  28. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  29. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  30. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
RRID
AB_2191072 (BioLegend Cat. No. 107625)
AB_2191071 (BioLegend Cat. No. 107626)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 10/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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