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PerCP/Cyanine5.5 anti-mouse CD198 (CCR8) Antibody

Pricing & Availability
Clone
SA214G2 (See other available formats)
Regulatory Status
RUO
Other Names
CDw198, C-C chemokine receptor type 8, CKR-8, CC-CKR-8, Ter1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SA214G2_PerCPCyanine5dot5_CD198_Antibody_020819_updated.png
C57BL/6 mouse thymocytes were stained with CD4 APC and CD198 (CCR8) (clone SA214G2) PerCP/Cyanine5.5 (left) or Rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right)
  • SA214G2_PerCPCyanine5dot5_CD198_Antibody_020819_updated.png
    C57BL/6 mouse thymocytes were stained with CD4 APC and CD198 (CCR8) (clone SA214G2) PerCP/Cyanine5.5 (left) or Rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right)
Compare all formats See PerCP/Cyanine5.5 spectral data
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150307 25 µg $176
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150308 100 µg $405
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Description

C-C chemokine receptor type 8 (CCR8) CD198, is a 41 kD G-protein coupled receptor with 7 transmembrane regions. CCR8 is expressed by a subset of thymocytes, Tregs, NKT and Th2-polarized cells, a subset of macrophages, monocytes, and monocyte-derived dendritic cells. CCR8 mediates chemotaxis toward its ligand CCL1, and is involved in apoptosis of thymocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD198 (CCR8)-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
RRID
AB_2572140 (BioLegend Cat. No. 150307)
AB_2572141 (BioLegend Cat. No. 150308)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 41 kD.
Distribution

Thymocytes, Tregs, NKT and Th2-polarized cells, subset of macrophages, monocytes and monocyte-derived dendritic cells.

Function
Chemotaxis, involved in thymocyte apoptosis
Ligand/Receptor
CCL1 (SCY1,TCA-3)
Cell Type
Dendritic cells, Macrophages, Monocytes, NKT cells, Th2, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Coghill JM, et al. 2013. Blood. 122:825.
2. Islam SA, et al. 2011. Nat. Immunol. 12:167.
3. Hoshino A, et al. 2007. J. Immunol. 178:5296.
3. Qu C, et al. 2004. J. Exp. Med. 200:1231.

Gene ID
12776 View all products for this Gene ID
UniProt
View information about CD198 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 02/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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