PerCP/Cyanine5.5 anti-mouse CD196 (CCR6) Antibody

Pricing & Availability
Clone
29-2L17 (See other available formats)
Regulatory Status
RUO
Other Names
CD196, BN-1, CKR-L3, DRY6, KY411, STRL22
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
29-2L17_PerCPCyanine5_CD196_Antibody_1_022324
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) APC and 29-2L17 PerCP/Cyanine5.5 (left) or Armenian Hamster IgG isotype control (right).
  • 29-2L17_PerCPCyanine5_CD196_Antibody_1_022324
    C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) APC and 29-2L17 PerCP/Cyanine5.5 (left) or Armenian Hamster IgG isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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129809 25 µg $188
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129810 100 µg $411
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Description

CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Product Citations
  1. Cortés JR, et al. 2022. Cell Rep. 39:110695. PubMed
  2. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
  3. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  4. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  5. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  6. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  7. Goode D, et al. 2014. PLoS Pathog. 10:1004567. PubMed
RRID
AB_2072797 (BioLegend Cat. No. 129809)
AB_2275515 (BioLegend Cat. No. 129810)

Antigen Details

Structure
G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6
Distribution

Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset

Function
Important in regulating mucosal immune response
Ligand/Receptor
CCL20/MIP-3a
Cell Type
B cells, Dendritic cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Rossi D, et al. 1997. J. Immunol. 158:1033.
2. Lukacs N, et al. 2001. J. Exp. Med. 194:551.
3. Cook DN, et al. 2000. Immunity 12:495

Gene ID
12458 View all products for this Gene ID
UniProt
View information about CD196 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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