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PerCP/Cyanine5.5 anti-human IgM Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHM-88_PerCPCyanine55_IgM_Antibody_080818
Overnight cultured human peripheral blood lymphocytes were stained with CD19 APC and anti-human IgM (clone MHM-88) PerCP/Cyanine5.5 (left), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • MHM-88_PerCPCyanine55_IgM_Antibody_080818
    Overnight cultured human peripheral blood lymphocytes were stained with CD19 APC and anti-human IgM (clone MHM-88) PerCP/Cyanine5.5 (left), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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314511 25 tests $176
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314512 100 tests $382
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
  3. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
  4. Català-Moll F, et al. 2021. Nucleic Acids Res. 49:5057. PubMed
  5. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  6. van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed
  7. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  8. Kohn L, et al. 2014. J Immunol. 192:5050. PubMed
  9. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  10. Aggarwal C, et al. 2021. J Virol. 95:e0061021. PubMed
  11. Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed
  12. van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed
  13. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  14. Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed
  15. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
  16. van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed
RRID
AB_961367 (BioLegend Cat. No. 314511)
AB_2076098 (BioLegend Cat. No. 314512)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 08/08/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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