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PerCP/Cyanine5.5 anti-human IgG Fc Recombinant Antibody

Pricing & Availability
Clone
QA19A42 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A42_PerCPCyanine5_IgG_Antibody_RECOM_092420
Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, then stained with anti-human IgG Fc (clone QA19A42) recombinant PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • QA19A42_PerCPCyanine5_IgG_Antibody_RECOM_092420
    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, then stained with anti-human IgG Fc (clone QA19A42) recombinant PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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366909 25 tests $223
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366910 100 tests $464
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Description

IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2876691 (BioLegend Cat. No. 366909)
AB_2876691 (BioLegend Cat. No. 366910)

Antigen Details

Structure
Homodimer formed by the constant region of the IgG heavy chain
Ligand/Receptor
CD16, CD32, CD64
Cell Type
B cells, Basophils, Dendritic cells, Macrophages, Mast cells, Neutrophils, NK cells, Platelets
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Innate Immunity
Molecular Family
Fc Receptors
Antigen References
  1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.
Gene ID
3500 View all products for this Gene ID
UniProt
View information about IgG Fc on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09/24/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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