PerCP/Cyanine5.5 anti-human HLA-A2 Antibody

Pricing & Availability
Clone
BB7.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class I
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BB7.2_PerCPCy5.5_HLA-A2_Antibody_FC_1_092812
Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) PerCP/Cyanine5.5 (filled histograms) or mouse IgG2b PerCP/Cyanine5.5 isotype control (open histograms).
  • BB7.2_PerCPCy5.5_HLA-A2_Antibody_FC_1_092812
    Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) PerCP/Cyanine5.5 (filled histograms) or mouse IgG2b PerCP/Cyanine5.5 isotype control (open histograms).
  • BB7.2_PerCPCy5.5_HLA-A2_Antibody_FC_2_092812
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
343315 25 tests $138
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343316 100 tests $358
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Description

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Papain solubilized HLA-A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
RRID
AB_2561572 (BioLegend Cat. No. 343315)
AB_2561573 (BioLegend Cat. No. 343316)

Antigen Details

Structure
Ig superfamily
Distribution

A*02 individuals leukocytes

Function
Antigen presentation
Ligand/Receptor
CD8, CD3/TCR
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 05/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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