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PerCP/Cyanine5.5 anti-human CD9 Antibody

Pricing & Availability
Clone
HI9a (See other available formats)
Regulatory Status
RUO
Workshop
V P018
Other Names
Tetraspanin, MRP-1, DRAP-24
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HI9a_PerCPCy55_CD9_Antibody_020518
Human platelets were stained with CD9 (clone HI9a) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • HI9a_PerCPCy55_CD9_Antibody_020518
    Human platelets were stained with CD9 (clone HI9a) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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312109 25 tests $141
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312110 100 tests $347
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Description

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rabbit, Rhesus, Sheep
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Yildizhan Y, et al. 2021. J Extracell Vesicles. e12059:10. PubMed
  2. Nazarova I, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Zhang C, et al. 2020. Front Oncol. 0.944444444. PubMed
RRID
AB_2728250 (BioLegend Cat. No. 312109)
AB_2728251 (BioLegend Cat. No. 312110)

Antigen Details

Structure
Tetraspan family, type III transmembrane protein, 24 kD
Distribution

Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells

Function
Adhesion, platelet activation, B cell development
Ligand/Receptor
Associates with CD63, CD81, CD82 and CD36, binds PSG17
Cell Type
B cells, Embryonic Stem Cells, Endothelial cells, Epithelial cells, Granulocytes, Lymphocytes, Platelets
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Miao WM, et al. 2001. Blood 97:1689.
2. Ellerman DA, et al. 2003. Mol Biol Cell. 14:5098.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

Gene ID
928 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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