PerCP/Cyanine5.5 anti-human CD79b (Igβ) Antibody

Pricing & Availability
Clone
CB3-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD79.1
Other Names
Igb, Igβ (Ig-beta), B29
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CB3-1_PerCPCyanine55_CD79b_Antibody_060619_updated.png
Human peripheral blood lymphocytes were stained with CD19 APC, and CD79b (Igβ) (clone CB3-1) PerCP/Cyanine5.5 (left), or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right)
  • CB3-1_PerCPCyanine55_CD79b_Antibody_060619_updated.png
    Human peripheral blood lymphocytes were stained with CD19 APC, and CD79b (Igβ) (clone CB3-1) PerCP/Cyanine5.5 (left), or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right)
Compare all formats See PerCP/Cyanine5.5 spectral data
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341407 25 tests $165
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341408 100 tests $358
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Description

CD79 is a heterodimeric molecule comprised of an α-chain (CD79a) and β-chain (CD79b). A 37-39 kD type I integral membrane protein CD79b is non-covalently associated with CD79a and cell surface IgM to form the B-cell receptor (BCR) complex. CD79b is expressed on the surface of surface Ig (sIg)-positive B cells and in the cytoplasm of sIg-negative B cells. It is essential for signal transduction after surface Ig crosslinking.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1992. P. Natl. Acad. Sci. USA 89:8522.
  2. Garcia Vela J, et al. 1999. Leukemia. 13:1501.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Rossi EA, et al. 2014. PLoS One. 9:98315. PubMed
Product Citations
  1. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
RRID
AB_1626196 (BioLegend Cat. No. 341407)
AB_2075869 (BioLegend Cat. No. 341408)

Antigen Details

Structure
37-39 kD type I integral membrane protein, associate with CD79a and cell surface IgM to form the B-cell receptor (BCR) complex, Ig superfamily.
Distribution

B cells

Function
From BCR complex, signal transduction
Cell Type
B cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc.
2. Dylke J, et al. 2007. Immunol. Lett. 112:47

Gene ID
974 View all products for this Gene ID
UniProt
View information about CD79b on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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