PerCP/Cy5.5 anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
Clone
C11.5 (See other available formats)
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C11.5_PerCPCy5.5_FC_1_092915
IFN-γ-primed and LPS-stimulated (overnight) human peripheral blood monocytes were surface stained with CD14 FITC and then intracellularly stained with IL-12/IL-23 p40 (clone C11.5) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).
  • C11.5_PerCPCy5.5_FC_1_092915
    IFN-γ-primed and LPS-stimulated (overnight) human peripheral blood monocytes were surface stained with CD14 FITC and then intracellularly stained with IL-12/IL-23 p40 (clone C11.5) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).
  • C11.5_PerCPCy5.5_FC_2_101315
See PerCP/Cy5.5 spectral data
Cat # Size Price Quantity Avail. Save
501821 25 tests $145
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501822 100 tests $325
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Description

The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.

Product Details
Technical data sheet

Product Details

Reactivity
Human, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CHO-expressed, recombinant human IL-12 p70 heterod
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Flow Cytometry3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization6: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813).
Additional reported applications (for the relevant formats) include:
Western blotting, ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4 of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
  2. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
  3. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
  4. Leifeld L, et al. 2002. Hepatology 36:1001.
  5. Pekkari K, et al. 2001. Blood 97:3184.
  6. Bolovan-Fritts CA, et al. 2004. J. Virol. 78:13173.
RRID
AB_2566055 (BioLegend Cat. No. 501821)
AB_2566056 (BioLegend Cat. No. 501822)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-?, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-? by human memory T cells, but not n
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells, PBL
Receptors
IL-12Rß1 binds p40; dimeric with IL-12Rß2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
3593 View all products for this Gene ID
UniProt
View information about IL-12 p40 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Go To Top Version: 2    Revision Date: 09/29/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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